Solid-state fermentation for the production of a recombinant beta-mannanase from Aspergillus fumigatus expressed in Aspergillus sojae grown on renewable resources

Yilmazer C., Germec M., TURHAN İ.

JOURNAL OF FOOD PROCESSING AND PRESERVATION, cilt.45, sa.8, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 8
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1111/jfpp.14584
  • Dergi Adı: JOURNAL OF FOOD PROCESSING AND PRESERVATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, not only beta (beta)-mannanase fabrication by recombinant Aspergillus sojae AsT3 in solid-state fermentation (SSF), but also the mannooligosaccharide (MOS) production was investigated. Barley husk, wheat bran, rye bran, oat husk, and spent coffee grounds were used as substrates. Plackett-Burman design was used to optimize SSF conditions (temperature, time, inoculation rate, and pH) based on enzyme activity and MOS results. While the highest beta-mannanase activity was found to be 983.53 U/mg in rye bran samples, it was found to be 582.59 U/mg, 55.59 U/mg, and 413.50 U/mg for the barley husk, wheat bran, and oat husk, respectively. The highest enzyme activity and MOS production were found to be 255.82 U/mg and 1,079.785 ppm with 7% inoculation rate, 30 degrees C, 7 days and pH 7. Results showed that SSF is suitable for beta-mannanase and MOS production due to the evaluation of wastes, lower energy requirement, and suitable environment for growth.