A quantitative polymerase chain reaction assay for the detection of equine (horse and donkey)-originated meat in processed bovine meat products

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ÖZÇELİK A., Yilmaz S., Gokbora S., İNAN M.

FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL, cilt.25, sa.1, ss.38-46, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1177/1082013218794849
  • Dergi Adı: FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.38-46
  • Anahtar Kelimeler: Equine, horse meat, donkey meat, species identification, quantitative polymerase chain reaction, authentication, REAL-TIME PCR, FRAGMENT LENGTH POLYMORPHISM, SPECIES IDENTIFICATION, CHICKEN, PORK, TURKEY, DNA, QUANTIFICATION, BEEF, MIXTURES
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Meat is one of the most important basic foodstuffs in human nutrition. Nowadays, adulteration and authenticity are common problems for meat products. Identification of meat species is important in terms of consumer protection and prevention of adulteration. There are different methods to determine adulteration of meat and meat products. These methods are histological controls, serological tests, and quantitative polymerase chain reaction. In this study, species identification and quantification analysis of meat and meat products were done by using horse-, donkey-, and bovine-specific primers with quantitative polymerase chain reaction method. Triple meat mixtures containing horse and donkey meat ranging from 0.1 to 50% levels were prepared within a bovine mixture for using species identification and quantification analysis. The method specificity was confirmed by melting curve analysis. In conclusion, quantitative polymerase chain reaction is an easy, rapid, and reliable method for meat species identification, and with this study an applicable method was developed for the detection and quantification of equine-originated meat in bovine meat products.