Effect of lactose hydrolysis on physicochemical and microbiological properties of probiotic yoghurt


Ergin F., Küçükçetin A.

V. International Joint Science Congress of Materials and Polymers, Burdur, Turkey, 29 September - 01 October 2021, pp.42-44, (Full Text)

  • Publication Type: Conference Paper / Full Text
  • City: Burdur
  • Country: Turkey
  • Page Numbers: pp.42-44
  • Akdeniz University Affiliated: Yes

Abstract

Lactose, which is a natural disaccharide formed by glucose and galactose, is exclusively found in mammalian

milk. Lactose is hydrolysed by enzymes named as lactase (β-galactosidase) and phlorizin hydrolase

(glycosyl-N-acylsphingosine glucohydrolase) in the health human small intestine. Lactose intolerance can be

caused by deficiency of β-galactosidase the brush-border of the small intestine and is defined as a clinical

syndromecharacterised by abdominal pain, flatulence, diarrhoea, nausea, and bloating that can occur after

lactose consumption. In recent years, the market for lactose-free or lactose-reduced dairy products has grown

considerably. In this study, milk with 50% and 100% lactose hydrolysis by β-galactosidase was used for

alternative lactose-reduced or lactose-free probiotic yoghurt. The control probiotic yoghurt samples were

produced without added β-galactosidase. The pH and titratable acidity values of the probiotic yoghurt samples

varied from 4.66 to 4.22 and from 0.92% to 1.27%, respectively, during the 30 days of storage. The apparent

viscosity and hardness values of the probiotic yoghurt samples produced from lactose hydrolysed milks were

lower than those of the control samples. The counts of Bifidobacterium bifidum, which was used as probiotic

bacteria in this study, in the probiotic yoghurt samples produced from milk with 50% and 100% lactose

hydrolysis and control samples ranged from 8.66 to 8.45 log cfu/g, 8.71 to 8.42 log cfu/g and 8.85 to 8.42 log

cfu/g, respectively, during the storage period. The counts of Streptococcus thermophilus and Lactobacillus

delbrueckii subsp. bulgaricus of the probiotic yoghurt samples produced from lactose hydrolysed milks were

slightly lower than those of the control samples.