Optimization of Antioxidant Activity and Phenolic Compound Extraction Conditions from Red Seaweed (Laurencia obtuse)

TOPUZ O. K., GÖKOĞLU N., Yerlikaya P., UÇAK I., GÜMÜŞ B.

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, vol.25, no.3, pp.414-422, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.1080/10498850.2013.868844
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.414-422
  • Keywords: Red seaweed, Laurencia obtuse, response surface methodology, phenolic compound extraction, antioxidants, ULTRASOUND-ASSISTED EXTRACTION, COMPONENTS, FRACTIONS, OXIDATION, CAPACITY, PLANTS, ASSAY
  • Akdeniz University Affiliated: Yes


Response surface methodology was used to investigate the effect of ultrasound-assisted extraction conditions including solvent:seaweed ratio (10:1-30:1), extraction temperature (30-50 degrees C), and extraction time (30-60 min) on the total phenolic compounds and antioxidant activity of red seaweed (Laurencia obtuse) extracts. The optimum extraction parameters for maximum phenolic content were as follows: solvent:seaweed ratio, 30:1; extraction temperature, 50 degrees C; and extraction time, 42.8 min. The experiment results showed that the solvent:seaweed ratio was the most significant parameter for the extraction. The optimum extraction conditions for maximum antioxidant activity were as follows: solvent:seaweed ratio, 24.3:1; extraction temperature, 45.3 degrees C; and extraction time, 58 min. Under the above-mentioned conditions, the experimental total phenolic content and antioxidant activity value were 26.23 +/- 0.75 mg GAE/g seaweed and 120.89 +/- 0.81 TEAC, respectively, of seaweed tested, which are well compatible with the predicted contents.