Optimization of Antioxidant Activity and Phenolic Compound Extraction Conditions from Red Seaweed (Laurencia obtuse)

TOPUZ O. K. , GÖKOĞLU N., Yerlikaya P., UÇAK I., GÜMÜŞ B.

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, vol.25, no.3, pp.414-422, 2016 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 3
  • Publication Date: 2016
  • Doi Number: 10.1080/10498850.2013.868844
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.414-422
  • Keywords: Red seaweed, Laurencia obtuse, response surface methodology, phenolic compound extraction, antioxidants, ULTRASOUND-ASSISTED EXTRACTION, COMPONENTS, FRACTIONS, OXIDATION, CAPACITY, PLANTS, ASSAY


Response surface methodology was used to investigate the effect of ultrasound-assisted extraction conditions including solvent:seaweed ratio (10:1-30:1), extraction temperature (30-50 degrees C), and extraction time (30-60 min) on the total phenolic compounds and antioxidant activity of red seaweed (Laurencia obtuse) extracts. The optimum extraction parameters for maximum phenolic content were as follows: solvent:seaweed ratio, 30:1; extraction temperature, 50 degrees C; and extraction time, 42.8 min. The experiment results showed that the solvent:seaweed ratio was the most significant parameter for the extraction. The optimum extraction conditions for maximum antioxidant activity were as follows: solvent:seaweed ratio, 24.3:1; extraction temperature, 45.3 degrees C; and extraction time, 58 min. Under the above-mentioned conditions, the experimental total phenolic content and antioxidant activity value were 26.23 +/- 0.75 mg GAE/g seaweed and 120.89 +/- 0.81 TEAC, respectively, of seaweed tested, which are well compatible with the predicted contents.