Optimization of Antioxidant Activity and Phenolic Compound Extraction Conditions from Red Seaweed (Laurencia obtuse)

TOPUZ O. K., GÖKOĞLU N., Yerlikaya P., UÇAK I., GÜMÜŞ B.

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, cilt.25, sa.3, ss.414-422, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/10498850.2013.868844
  • Dergi Adı: JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.414-422
  • Anahtar Kelimeler: Red seaweed, Laurencia obtuse, response surface methodology, phenolic compound extraction, antioxidants, ULTRASOUND-ASSISTED EXTRACTION, COMPONENTS, FRACTIONS, OXIDATION, CAPACITY, PLANTS, ASSAY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Response surface methodology was used to investigate the effect of ultrasound-assisted extraction conditions including solvent:seaweed ratio (10:1-30:1), extraction temperature (30-50 degrees C), and extraction time (30-60 min) on the total phenolic compounds and antioxidant activity of red seaweed (Laurencia obtuse) extracts. The optimum extraction parameters for maximum phenolic content were as follows: solvent:seaweed ratio, 30:1; extraction temperature, 50 degrees C; and extraction time, 42.8 min. The experiment results showed that the solvent:seaweed ratio was the most significant parameter for the extraction. The optimum extraction conditions for maximum antioxidant activity were as follows: solvent:seaweed ratio, 24.3:1; extraction temperature, 45.3 degrees C; and extraction time, 58 min. Under the above-mentioned conditions, the experimental total phenolic content and antioxidant activity value were 26.23 +/- 0.75 mg GAE/g seaweed and 120.89 +/- 0.81 TEAC, respectively, of seaweed tested, which are well compatible with the predicted contents.