TOMATO BROWN RUGOSE FRUIT VIRUS (ToBRFV)'ÜN TOHUM ÜZERİNDE TAŞINMA ORANI, TAŞINMA YERİ VE ERADİKASYON İŞLEMLERİ


Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2022

Tezin Dili: Türkçe

Öğrenci: DAMLA ULUSOY

Danışman: Hakan Fidan

Özet:

Reasons such as the increase in global seed trade and the facilitation of

international transportation ensure the rapid spread of newly emerged viruses and the

spread of diseases to clean areas. One of these viruses is Tomato brown rugose fruit virus

(ToBRFV) disease, which has gained much importance especially in recent years.

ToBRFV, It is a member of the Tobamovirus group, which also includes plant viruses

such as TMV and ToMV. While ToBRFV is transmitted by seeds over long distances, it

can spread very easily by contact between short distances. Active seed trade and the

importance of the region we are in in agriculture are the reasons that make the

transmission of ToBRFV by seed dangerous. This situation has brought the eradication

studies against the viruses found in the seeds to the fore in the fight against ToBRFV.

Within the scope of this thesis, some chemical and physical methods applied in

the disinfection of seeds were examined. Of the physical methods, the virus density was

found to be the least in seeds subjected to heat treatment at 72 °C for 72 hours. It was

found that in the application of heat treatment and UV-C applications (254 nm), the virus

loses its pathogenicity. According to the information obtained as a result of different

disinfectant applications to seeds: 1% Tsunami 100, 1% Biocon A and 10% TSP have

been determined as the optimal methods of combating this virus. In addition to the success

of seed eradication, these methods do not adversely affect germination. These studies

aimed at limiting the spread of the virus will make an important contribution to domestic

and national seed production.