Antalya İli Patlıcan Yetiştiriciliğinde Sorun Olan Virüs Hastalıklarının Belirlenmesi ve En Yaygın Virüsün Moleküler Karakterizasyonu


Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, Türkiye

Tezin Onay Tarihi: 2019

Tezin Dili: Türkçe

Öğrenci: Pelin Sarıkaya

Asıl Danışman (Eş Danışmanlı Tezler İçin): Hakan Fidan

Özet:

This study was carried out because of the fact that there was no interest searchin the detection of the virus diseases in the eggplant lands in Antalya province of Mediterranean region, which is the leader in eggplant production in Turkey. Between 2016-2018, surveys were conducted in the districts of Antalya province and samples were collected according to the production quantities. The samples were tested against Eggplant mottled dwarf virus (EMDV) and Tomato spotted wilt virus (tswv) using DAS-ELISA method. The PCR method is tested against Tomato yellow leaf curl virus as well as Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV),  Eggplant mosaic virus (EMV) (=Andean potato latent virus) (APLV), Eggplant mottled crinkle virus (EMCV), Eggplant mottled dwarf virus (EMDV), Potato Y virus (PVY), Tomato bushy stunt virus (TBSV), Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tomato chlorosis virus (ToCV) and Tomato spotted wilt virus (TSWV) with the RT-PCR method. Results showed no infective samples with AMV, APLV, EMCV, PVY, TBSV and TMV. Among the samples; %10 were found to be infected with ToCV, %6 with ToMV, %5 with EMDV, %4 with TSWV and %3 with CMV. TYLCV was found to be the most common virus by 19%. For this reason, TYLCV's races are determined and characterized by bioinformatics. Samples with infected by TYLCV were found %40 with Tomato yellow leaf curl virus Mild and %60 with Tomato yellow leaf curl virus  Israel.