TOMATO BROWN RUGOSE FRUIT VIRUS (TOBRFV) DOMATES BİTKİSİNE BULAŞMASI VE SERA ALET EKİPMANLARI ÜZERİNDE AKTİF KALMA SÜRELERİ İLE İLGİLİ ÇALIŞMALAR VE ERADİKASYON İŞLEMLERİ


Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye

Tezin Onay Tarihi: 2022

Tezin Dili: Türkçe

Öğrenci: HANDE NUR ALBEZİRGAN

Danışman: Hakan Fidan

Özet:

The climate changes experienced by the world, as well as the rapid spread of emerging viruses, conditions that were previously unfavorable for the disease become suitable for the disease and cause it to reach clean areas where the disease does not exist. One of these viruses is Tomato brown rugose fruit virus, which was reported in our country in 2019. Among the factors limiting the production of tomato plants in our country, ToBRFV, which has become widespread in the last few years, can easily spread by contact between short distances while it is carried by seed between long distances. The fact that ToBRFV, which is known as a virus easily transmitted by mechanical contact, can infect the seed and be transported by the seed has increased the importance of this virus even more. The active trade of seeds in our country and the fact that the Antalya region is the center of this area is dangerous in terms of the spread of the disease in this region. ToBRFV is a member of the Tobamovirus group, which includes plant viruses such as TMV (Tobacco mosaic virus) and ToMV (Tomato mosaic virus). The best control method against viral diseases that do not have a chemical control is to develop resistant varieties. However, the Tm22 gene, which provides resistance to TMV and ToMV in plants today, does not provide resistance to ToBRFV. For this reason, studies to prevent ToBRFV from infecting tomato production areas are very important. Since it is a newly detected virus, there is no detailed study on it. The absence of a resistant variety and the absence of chemical control have highlighted the efforts to protect and recognize the virus.

ToBRFV reported in Turkey in 2019 greatly damages fruit yield and quality in tomato growing areas. Since commercially produced tomato varieties do not have resistance to ToBRFV, it is important to take hygiene measures during the production season in the cultivation area. Within the scope of the study, the effectiveness of disinfectants was investigated. Disinfectants, which are widely used in seed growing, nursery and greenhouse production areas, were preferred. Trials were conducted to determine the effectiveness of disinfectants against ToBRFV under controlled and farmer conditions. The experiment continued from seed to seedling period and then from planting to greenhouse until the end of harvest, taking autumn tomato cultivation as an example. In this process, the active duration of ToBRFV on the surfaces of tools and equipment used during cultural processes was determined.

BioconA, Bioxi and Tsunami were found to be effective in evaluating the effectiveness of disinfectants against ToBRFV under controlled conditions. In the evaluation of the effectiveness of disinfectants against ToBRFV in farmer conditions, all plants in the greenhouse were found to be 100% ToBRFV contaminated at the end of the experiment, although disinfectants applied with hand sprayers from the top delayed the contamination. In the determination of the active time of disinfectants in the greenhouse tools and equipment, it was determined that the most active time on the metal surface was found to be active on the metal surface, while it remained active on the latex glove and binding thread the lowest.