Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye
Tezin Onay Tarihi: 2022
Tezin Dili: Türkçe
Öğrenci: HANDE NUR ALBEZİRGAN
Danışman: Hakan Fidan
Özet:
The
climate changes experienced by the world, as well as the rapid spread of
emerging viruses, conditions that were previously unfavorable for the disease
become suitable for the disease and cause it to reach clean areas where the
disease does not exist. One of these viruses is Tomato brown rugose fruit
virus, which was reported in our country in 2019. Among the factors limiting
the production of tomato plants in our country, ToBRFV, which has become
widespread in the last few years, can easily spread by contact between short
distances while it is carried by seed between long distances. The fact that
ToBRFV, which is known as a virus easily transmitted by mechanical contact, can
infect the seed and be transported by the seed has increased the importance of
this virus even more. The active trade of seeds in our country and the fact
that the Antalya region is the center of this area is dangerous in terms of the
spread of the disease in this region. ToBRFV is a member of the Tobamovirus
group, which includes plant viruses such as TMV (Tobacco mosaic virus) and ToMV
(Tomato mosaic virus). The best control method against viral diseases that do
not have a chemical control is to develop resistant varieties. However, the Tm22
gene, which provides resistance to TMV and ToMV in plants today, does not
provide resistance to ToBRFV. For this reason, studies to prevent ToBRFV from
infecting tomato production areas are very important. Since it is a newly
detected virus, there is no detailed study on it. The absence of a resistant
variety and the absence of chemical control have highlighted the efforts to
protect and recognize the virus.
ToBRFV reported in Turkey in
2019 greatly damages fruit yield and quality in tomato growing areas. Since
commercially produced tomato varieties do not have resistance to ToBRFV, it is
important to take hygiene measures during the production season in the
cultivation area. Within the scope of the study, the effectiveness of
disinfectants was investigated. Disinfectants, which are widely used in seed
growing, nursery and greenhouse production areas, were preferred. Trials were
conducted to determine the effectiveness of disinfectants against ToBRFV under
controlled and farmer conditions. The experiment continued from seed to
seedling period and then from planting to greenhouse until the end of harvest,
taking autumn tomato cultivation as an example. In this process, the active
duration of ToBRFV on the surfaces of tools and equipment used during cultural
processes was determined.
BioconA, Bioxi and Tsunami were
found to be effective in evaluating the effectiveness of disinfectants against
ToBRFV under controlled conditions. In the evaluation of the effectiveness of
disinfectants against ToBRFV in farmer conditions, all plants in the greenhouse
were found to be 100% ToBRFV contaminated at the end of the experiment,
although disinfectants applied with hand sprayers from the top delayed the
contamination. In the determination of the active time of disinfectants in the
greenhouse tools and equipment, it was determined that the most active time on
the metal surface was found to be active on the metal surface, while it
remained active on the latex glove and binding thread the lowest.