Molecular Characterization of Some Selected Landrace Green Bean (Phaseolus vulgaris L.) Genotypes

ULUKAPI K., ONUS A. N.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.18, sa.4, ss.277-286, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1501/tarimbil_0000001216
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.277-286
  • Anahtar Kelimeler: Green bean, Molecular analysis, SSR, SCAR, Genetic relationship, ANGULAR LEAF-SPOT, GENETIC DIVERSITY, SSR MARKERS, IDENTIFICATION, RESISTANCE, MODEL, SPP.
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Molecular markers are widely used to investigate the genetic diversity among bean species. This study was conducted to reveal the molecular characterization of 33 dwarf green bean landraces selected by Karadeniz Agricultural Research Institute. Genotypes were evaluated using Sequence Characterized Amplified Region (SCAR) and Simple Sequence Repeat (SSR) markers. Approximately 73% of the SSR markers gave polymorphic bands and the polymorphism information content (PIC) was between 0.047 and 0.373. All of the SCAR markers were polymorphic and the PIC showed variation from 0.071 to 0.379. Genetic similarity index among green bean genotypes varied between 0.52 and 0.98. Principle coordinate analysis indicated that genetic diversity among the genotypes was not high and all selected genotypes had a close genetic relationship with Andean gene pool.