Akademik Veri Yönetim Sistemi
3rd International Multidisciplinary Cancer Research Congress, İstanbul, Türkiye, 7 - 10 Eylül 2023, ss.70
Introduction and Aim: Many herbal compounds are applied for phytotherapy purposes in lung cancer (H1299), the incidence of which is increasing globally. However, it is a fact that this compound, which will be used to treat lung cancer, also affects healthy cells. Activation of the apoptotic pathway was investigated to demonstrate the anticancer effect of catechol on drug-resistant lung cancer cells. In addition, the activation of the apoptotic pathway was studied with healthy fibroblast (Bj) cells and the results were compared.
Materials and Methods: The 24-hour cytotoxic effect of catechol on cells was demonstrated by the Cell Titer-Blue® cell viability test. In order to determine the apoptotic potential, the caspase 3/7 activity of the cells exposed to catechol was measured and the potential of catechol to stimulate the apoptotic pathway was determined. Caspase 3/7 enzyme activity was determined using the Promega 'ApoTox-GloTM Triplex Assay' kit.
Results: In our study, the IC50 values of the cytotoxic effect of catechol on drug-resistant lung cancer and healthy fibroblast cells were found to be 90 and 207 μg/ml, respectively. Caspase-3/7 activity in drug-resistant lung cancer and Bj cells increased 2.3-fold and 1.6-fold, respectively, compared to control after 24 hours of catechol incubation.
Conclusion: In conclusion, drug-resistant lung cancer cells have higher caspase 3 activity than healthy cells, indicating that catechol has a higher apoptotic potential in drug-resistant cancer cells than healthy cells.
Keywords: Catechol, Drug-resistant, Apoptosis
Acknowledgement and/or disclaimers if any: The authors wish to thanks to Akdeniz University BAP-Scientific Research Projects Coordination Unit (FYL-2022-5902) for financial support of this work.