Mapping quantitative trait loci and developing first molecular marker for race 5 of Podosphera xanthii resistance in melon (Cucumis melo L.)


Ünlü A., Polat İ., Yıldırım A., Onus A. N.

TURKISH JOURNAL OF BOTANY, cilt.46, sa.0, ss.123-133, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 0
  • Basım Tarihi: 2022
  • Dergi Adı: TURKISH JOURNAL OF BOTANY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Geobase, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.123-133
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Powdery mildew of melon caused by the Podosphaera xanthii factor is a disease that causes serious yield losses economically

in all areas where melon is grown from Asia to Europe and America. The use of resistant varieties for the control of the disease is the

most environmentally friendly method. However, traditional methods of melon breeding have made a noteworthy input to resistance

varieties improvement, but they have been slow and incompetent in targeting complex powdery mildew disease resistance traits. To

overcome these problems and to facilitate breeding programs, molecular markers closely linked to the Pm-R5 gene are needed for

marker-assisted selection (MAS). The present study aimed to map quantitative trait locus for powdery mildew resistance and identify

the molecular markers linked with the Pm-R gene which provides resistance to race 5 of P. xanthii for MAS breeding in melons. A total

of 310 primers/combinations belonging to six marker systems including SRAP, SSR, ISSR, TRAP, CAPS, SCAR in the RIL population

consisting of 136 genotypes, developed from crosses PMR-6 and susceptible inbred line were used for QTL mapping. Quantitative trait

loci analysis determined one major QTL (Pm-R) on linkage group I for resistance to races 5 of powdery mildew. The SSR12202 marker

is linked to the Pm-R gene QTL at 2.12 cM genetic distance, and the CMCT170b and CMMS30-3 markers are determined in the support

interval of this QTL. The co-dominant markers, together with the map information reported here could be used for genotyping selection

of resistance to race 5 of P. xanthii in melon breeding.