TURKISH JOURNAL OF BOTANY, cilt.46, sa.0, ss.123-133, 2022 (SCI-Expanded)
Powdery mildew of melon caused by the Podosphaera xanthii factor is a disease that causes serious yield losses economically
in all areas where melon is grown from Asia to Europe and America. The use of resistant varieties for the control of the disease is the
most environmentally friendly method. However, traditional methods of melon breeding have made a noteworthy input to resistance
varieties improvement, but they have been slow and incompetent in targeting complex powdery mildew disease resistance traits. To
overcome these problems and to facilitate breeding programs, molecular markers closely linked to the Pm-R5 gene are needed for
marker-assisted selection (MAS). The present study aimed to map quantitative trait locus for powdery mildew resistance and identify
the molecular markers linked with the Pm-R gene which provides resistance to race 5 of P. xanthii for MAS breeding in melons. A total
of 310 primers/combinations belonging to six marker systems including SRAP, SSR, ISSR, TRAP, CAPS, SCAR in the RIL population
consisting of 136 genotypes, developed from crosses PMR-6 and susceptible inbred line were used for QTL mapping. Quantitative trait
loci analysis determined one major QTL (Pm-R) on linkage group I for resistance to races 5 of powdery mildew. The SSR12202 marker
is linked to the Pm-R gene QTL at 2.12 cM genetic distance, and the CMCT170b and CMMS30-3 markers are determined in the support
interval of this QTL. The co-dominant markers, together with the map information reported here could be used for genotyping selection
of resistance to race 5 of P. xanthii in melon breeding.