First report of Tomato leaf curl New Delhi virus in Türkiye

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Fidan H., Yıldız K., Sarıkaya P., Çalış Ö.

New Disease Reports, vol.47, no.2, pp.1-2, 2023 (SCI-Expanded)

  • Publication Type: Article / Case Report
  • Volume: 47 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.1002/ndr2.12180
  • Journal Name: New Disease Reports
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-2
  • Akdeniz University Affiliated: Yes

Abstract

Aleafcurldiseasehasbeenobservedfrequentlyingreenhouseswherecucumber(Cucumis sativus),melon(Cucumis melo)andsquash(Cucur-bita pepo) are produced in Antalya since 2019. The infected plantsshowed symptoms including apical leaf curl, vein swelling, severelystunted,wrinkledleaves,fruitshapeandtexturedisorderandacon-spicuousmosaicpattern(Figure1–5).ThesesymptomsaresimilartothosecausedbyTomato leaf curl New Delhi virus(ToLCNDV),animpor-tantmemberofthegenusBegomovirus,familyGeminiviridae.ToLCNDVistransmittedbyBemisia tabaciinapersistentcirculativemanner,caus-ingseriousdamagetoawidevarietyofplantspecies,especiallyintheSolanaceaeandCucurbitaceaefamilies(Briddon,2015).ToLCNDVwasfirstidentifiedonsolanaceouscropsinIndia(Padidametal.,1995)butthe virus is recognised as an emerging threat worldwide due to theFIGURE1TypicalTomato leaf curl New Delhi virussymptomsonsquashleavesThisisanopenaccessarticleunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsuse,distributionandreproductioninanymedium,providedtheoriginalworkisproperlycited.©2023TheAuthors.New Disease ReportspublishedbyBritishSocietyforPlantPathologyandJohnWiley&Sons.severityofthediseaseanditsincreasingprevalence.OutbreakshaverecentlyoccurredincountriesinAfrica,AsiaandEurope(EuropeanandMediterraneanPlantProtectionOrganization,2023).SampleswerecollectedfromeightadjoinedgreenhousesinAntalya,fourgrowingcucumber,twomelonandtwosquash.Threeleafsamplesandonefruitsamplewerecollectedindividuallyfromadiseasedplantineachofthegreenhouses,thatis32samplesintotal.TotalnucleicacidwasextractedfromtheleafandfruitsamplesandanalysedbyPCRwithspecificToLCNDVprimers(ToA1-FandToA1-R)whichamplifya504bpfragmentofviralDNA-A(Saezetal.,2016).Thefollowingcon-ditionswereusedforPCR:95◦Cfor5min;35cyclesof30sat95◦C,45sat55◦Cand45sat72◦C,followedbyafinal72◦Cextensionstepfor10min.SequenceswereobtainedfromsamplesfromeachoftheFIGURE2TypicalTomato leaf curl New Delhi virussymptomsonsquashfruitsNew Dis Rep.2023;47:e12180.wileyonlinelibrary.com/journal/ndr21of2https://doi.org/10.1002/ndr2.12180