A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components
TURKISH JOURNAL OF BIOLOGY, cilt.35, sa.6, ss.735-742, 2011 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 35 Sayı: 6
- Basım Tarihi: 2011
- Doi Numarası: 10.3906/biy-1011-170
- Dergi Adı: TURKISH JOURNAL OF BIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.735-742
- Anahtar Kelimeler: O. onites, essential oil, antioxidant, anticancer, EXTRACTS, L., ANTIBACTERIAL, THYMOL, PLANTS, WILD
- Akdeniz Üniversitesi Adresli: Evet
Özet
The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum lutes L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential oil of O. onites decreased cancer cell viability at higher concentrations. The essential oil was found to be less cytototoxic (IC50: 149.12 mu g/mL) than carvacrol and thymol (IC50: 53.09 and 60.01 mu g/mL, respectively) for Hep G2 cells. On the other hand, with the O. onites essential oil, carvacrol and thymol protected the cells against H2O2-induced cytotoxicity when the cells were preincubated with the oil and its components at a lower concentration (