Akademik Veri Yönetim Sistemi
15th International Congress of the Innovative Agricultural Technologies, IAT 2025, Antalya, Türkiye, 15 - 19 Ekim 2025, cilt.805 LNCE, ss.14-27, (Tam Metin Bildiri)
The olive oil industry is a major global agricultural sector, generating large amounts of olive tree pruning waste (OTPW) as a by-product of annual pruning practices. This residue, averaging 3 tons per hectare, is often burned or left in the field, leading to environmental concerns. Valorization of OTPW through biorefinery approaches offers opportunities for producing value-added bio-based products. In this study, OTPW was subjected to four pretreatment methods: (i) deep eutectic solvent (DES; choline chloride:formic acid, 1:2 molar ratio), (ii) dilute acid (2.5% H₂SO₄), (iii) alkaline (2.5% NaOH), and (iv) autohydrolysis. DES, acid, and alkaline pretreatments were conducted at 90–130 °C for 20–60 min, while autohydrolysis was performed at 150–190 °C for 10–30 min. Solid recovery varied between 87% and 41%, decreasing with severity except in alkaline pretreatment. Among the tested methods, DES pretreatment achieved the highest total phenolic content (144.5 mg GAE/g dry biomass), highlighting its efficiency for phenolic extraction. Acid pretreatment yielded the highest monosaccharide concentrations (11 g/L xylose, 7.5 g/L glucose), making it suitable for fermentable sugar production. Autohydrolysis was the most effective for xylo-oligosaccharide (XOS) generation, achieving up to 11.3 g/100 g biomass at 190 °C for 10 min, underscoring its potential for prebiotic applications. The results demonstrate that pretreatment type strongly influences the selective recovery of valuable compounds. These findings emphasize the role of tailored pretreatment strategies in enabling integrated biorefinery concepts, supporting the sustainable valorization of OTPW for biofuels, functional ingredients, and bioactive compounds.