A novel root-specific gene, MIC-3, with increased expression in nematode-resistant cotton (Gossypium hirsutum L.) after root-knot nematode infection

ZHANG X., CALLAHAN F., JENKINS J., MA D., Karaca M., SAHA S., ...More

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, vol.1576, no.1-2, pp.214-218, 2002 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1576 Issue: 1-2
  • Publication Date: 2002
  • Doi Number: 10.1016/s0167-4781(02)00309-3
  • Journal Name: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.214-218
  • Keywords: cotton, nematode, host resistance, root gene expression, alternative polyadenylation, MESSENGER-RNA SEQUENCES, SITES, IDENTIFICATION, TRANSCRIPTION, PENETRATION, TOMATO
  • Akdeniz University Affiliated: Yes

Abstract

A full-length cDNA, MIC-3, has been identified from a lambdaZAPII cDNA library constructed from the mRNA of nematode-resistant cotton (Gossypium hirsutum L.) roots after infection with root-knot nematode (Meloidogyne incognita). The putative open reading frame of MIC-3 encoded a protein of 141 amino acids with a calculated molecular mass of 15.3 kDa. Seven alternative polyadenylation sites have been identified for the MIC-3 transcripts, and the major transcripts are the longest ones. The MIC-3 gene contains a single intron within its coding region and belongs to a novel, multi-gene family containing up to six members. Expression of MIC-3 is root localized and specifically enhanced in the nematode induced, immature galls of resistant cotton line M-249, suggesting that MIC-3 may play a critical role in the resistance response to root-knot nematode. Published by Elsevier Science B.V.