A novel root-specific gene, MIC-3, with increased expression in nematode-resistant cotton (Gossypium hirsutum L.) after root-knot nematode infection

ZHANG X., CALLAHAN F., JENKINS J., MA D., Karaca M., SAHA S., ...Daha Fazla

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, cilt.1576, sa.1-2, ss.214-218, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1576 Sayı: 1-2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0167-4781(02)00309-3
  • Dergi Adı: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.214-218
  • Anahtar Kelimeler: cotton, nematode, host resistance, root gene expression, alternative polyadenylation, MESSENGER-RNA SEQUENCES, SITES, IDENTIFICATION, TRANSCRIPTION, PENETRATION, TOMATO
  • Akdeniz Üniversitesi Adresli: Evet

Özet

A full-length cDNA, MIC-3, has been identified from a lambdaZAPII cDNA library constructed from the mRNA of nematode-resistant cotton (Gossypium hirsutum L.) roots after infection with root-knot nematode (Meloidogyne incognita). The putative open reading frame of MIC-3 encoded a protein of 141 amino acids with a calculated molecular mass of 15.3 kDa. Seven alternative polyadenylation sites have been identified for the MIC-3 transcripts, and the major transcripts are the longest ones. The MIC-3 gene contains a single intron within its coding region and belongs to a novel, multi-gene family containing up to six members. Expression of MIC-3 is root localized and specifically enhanced in the nematode induced, immature galls of resistant cotton line M-249, suggesting that MIC-3 may play a critical role in the resistance response to root-knot nematode. Published by Elsevier Science B.V.