A Novel Molecularly Imprinting Biosensor Including Graphene Quantum Dots/Multi-Walled Carbon Nanotubes Composite for Interleukin-6 Detection and Electrochemical Biosensor Validation

ÖZCAN N., KARAMAN C., ATAR N., KARAMAN O., Yola M. L.

ECS JOURNAL OF SOLID STATE SCIENCE AND TECHNOLOGY, cilt.9, sa.12, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1149/2162-8777/abd149
  • Dergi Adı: ECS JOURNAL OF SOLID STATE SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Compendex, INSPEC
  • Anahtar Kelimeler: Interleukin-6, graphene quantum dots, multi-walled carbon nanotubes, biosensor validation, BORON-NITRIDE NANOSHEETS, LABEL-FREE, AU NANOPARTICLES, FACILE SYNTHESIS, DOTS, CANCER, POLYMER, SENSOR, HYBRID, OXIDE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Interleukin-6 (IL-6) as a pro-inflammatory cytokine demonstrate a critical role in the inflammatory response. Especially, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. In this report, new molecularly imprinting biosensor on graphene quantum dots (GQDs)/functionalized multi-walled carbon nanotubes (f-MWCNTs) composite were prepared for IL-6 protein detection. The structures of GQDs, f-MWCNTs and GQDs/f-MWCNTs composite were highlighted by scanning electron microscope (SEM), transmission electron microscopy (TEM), raman spectroscopy, UV-vis spectroscopy, fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and X-ray diffraction (XRD) method. Surface morphology characterization shows the nanoporous cavities as an effective biosensing area. IL-6 protein imprinted electrode was prepared on GQDs/f-MWCNTs composite in the presence of 100.0 mM pyrrole containing 25.0 mM IL-6 protein. 0.01-2.0 pg ml(-1) and 0.0030 pg ml(-1) were found as linearity range and the detection limit (LOD) for analytical application in plasma samples. Finally, the validated biosensor was examined in terms of stability, repeatability and reproducibility.