The effect of mild hypothermia, mannitol and insulin-induced hypoglycaemia on ischaemic infarct volume in the early period after permanent middle cerebral artery occlusion in the rat

Kazan S., Karasoy M., Baloglu H., Tuncer R.

ACTA NEUROCHIRURGICA, cilt.141, sa.9, ss.979-987, 1999 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 141 Sayı: 9
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1007/s007010050405
  • Dergi Adı: ACTA NEUROCHIRURGICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.979-987
  • Anahtar Kelimeler: hypoglycaemia, hypothermia, insulin, ischaemia, mannitol, rat, TEMPORARY VESSEL OCCLUSION, BLOOD-FLOW, FOCAL ISCHEMIA, INTRAISCHEMIC HYPOTHERMIA, BRAIN TEMPERATURE, NEURONAL INJURY, RISK-FACTORS, REPERFUSION, GLUCOSE, STROKE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

We investigated the effect of mild hypothermia (32-34 degrees C), mannitol and insulin - induced hypoglycaemia on the ischaemic infarct volume on permanent middle cerebral artery occlusion with bilateral carotid artery ligation in rats. Temporalis muscle temperature as an indicator of brain temperature was monitored throughout the experiment in all rats, which were randomly divided into seven groups. During ischaemia, control rats received intravenous saline in a normothermic condition; treated rats had hypothermia and intravenous saline, hypothermia and mannitol, normothermia and mannitol, normothermia and insulin, normothermia, insulin and glucose, and hypothermia and insulin applied. After the experiment, the animals were killed, and brain sections were stained with haematoxylin and eosin. Images of infarct areas were determined using a camera attached to the microscope, and analysed by image analysis software. The total volume of infarcted tissue, right hemispheric volume, and the percentage of infarction were determined at the end of the image analysis investigation.