The effect of mild hypothermia, mannitol and insulin-induced hypoglycaemia on ischaemic infarct volume in the early period after permanent middle cerebral artery occlusion in the rat

Kazan S., Karasoy M., Baloglu H., Tuncer R.

ACTA NEUROCHIRURGICA, vol.141, no.9, pp.979-987, 1999 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 141 Issue: 9
  • Publication Date: 1999
  • Doi Number: 10.1007/s007010050405
  • Journal Name: ACTA NEUROCHIRURGICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.979-987
  • Keywords: hypoglycaemia, hypothermia, insulin, ischaemia, mannitol, rat, TEMPORARY VESSEL OCCLUSION, BLOOD-FLOW, FOCAL ISCHEMIA, INTRAISCHEMIC HYPOTHERMIA, BRAIN TEMPERATURE, NEURONAL INJURY, RISK-FACTORS, REPERFUSION, GLUCOSE, STROKE
  • Akdeniz University Affiliated: Yes

Abstract

We investigated the effect of mild hypothermia (32-34 degrees C), mannitol and insulin - induced hypoglycaemia on the ischaemic infarct volume on permanent middle cerebral artery occlusion with bilateral carotid artery ligation in rats. Temporalis muscle temperature as an indicator of brain temperature was monitored throughout the experiment in all rats, which were randomly divided into seven groups. During ischaemia, control rats received intravenous saline in a normothermic condition; treated rats had hypothermia and intravenous saline, hypothermia and mannitol, normothermia and mannitol, normothermia and insulin, normothermia, insulin and glucose, and hypothermia and insulin applied. After the experiment, the animals were killed, and brain sections were stained with haematoxylin and eosin. Images of infarct areas were determined using a camera attached to the microscope, and analysed by image analysis software. The total volume of infarcted tissue, right hemispheric volume, and the percentage of infarction were determined at the end of the image analysis investigation.