Acute and Chronic Exposure to 900 MHz Radio Frequency Radiation Activates p38/JNK-mediated MAPK Pathway in Rat Testis.

Er H., Tas G. G., Soygur B., Ozen Ş., Sati L.

Reproductive sciences (Thousand Oaks, Calif.), cilt.29, sa.5, ss.1471-1485, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s43032-022-00844-y
  • Dergi Adı: Reproductive sciences (Thousand Oaks, Calif.)
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, MEDLINE
  • Sayfa Sayıları: ss.1471-1485
  • Anahtar Kelimeler: Radio frequency radiation, Rat, Testis, p38, JNK, OXIDATIVE STRESS, MOBILE PHONE, RADIOFREQUENCY RADIATION, ELECTROMAGNETIC-FIELD, PROTEIN-KINASES, DIELECTRIC-PROPERTIES, MOLECULAR-MECHANISMS, MALE-INFERTILITY, DNA-DAMAGE, GERM-CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The use of electronic devices such as mobile phones has had a long stretch of rapid growth all over the world. Therefore, exposure to radio frequency radiation (RFR) has increased enormously. Here, we aimed to assess the balance between cell death and proliferation and also investigate the involvement of the JNK/p38 MAPK signaling pathway in the testis of rats exposed to 900 MHz RFR in acute and chronic periods (2 h/day, 5 days/week) for 1 or 10 weeks, respectively. The expression of proliferating cell nuclear antigen (PCNA), Bcl-xL, cleaved caspase-3, phosphorylated-JNK (p-JNK), and phosphorylated-p38 (p-p38) was analyzed in line with histopathology and TUNEL analysis in rat testis. There were no histopathological differences between sham and RFR groups in the acute and chronic groups. PCNA expression was not altered between groups in both periods. However, alterations for cleaved caspase-3 and Bcl-xL were observed depending on the exposure period. TUNEL analysis showed a significant increase in the RFR group in the acute period, whereas no difference in the chronic groups for the apoptotic index was reported. In addition, both p-p38 and p-JNK protein expressions increased significantly in RFR groups in both periods. Our study indicated that 900 MHz RFR might result in alterations during acute period exposure for several parameters, but this can be ameliorated in the chronic period in rat testis. Here, we also report the involvement of the p38/JNK-mediated MAPK pathway after exposure to 900 MHz RFR. Hence, this information might shed light in future studies toward detailed molecular mechanisms in male reproduction and infertility.