Association of interleukin 18-607A/C and -137C/G polymorphisms with oxidative stress in renal transplant recipients.

Creative Commons License

Davran F., Yilmaz V. T., Erdem B., Gultekin M., Suleymanlar G., Akbas H.

Renal failure, cilt.38, ss.717-22, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3109/0886022x.2016.1158034
  • Dergi Adı: Renal failure
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.717-22
  • Anahtar Kelimeler: Interleukin-18 gene polymorphisms, oxidative stress, renal transplantation, LIPID-PEROXIDATION, KIDNEY, ABNORMALITIES, INFLAMMATION, ACTIVATION, PATHWAY, INJURY, CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objectives IL-18 mediates various inflammatory and oxidative responses including renal injury, fibrosis, and graft rejection. It has been reported that the promoter -607 and -137 polymorphisms of IL-18 influence the level of IL-18. This prospective observational study investigated the association between oxidative stress with IL-18-607 and -137 polymorphisms in renal transplant recipients. Patients and methods This study included 75 renal transplant recipients (28 female, 47 male) from living-related donors. Blood samples were collected immediately before and after transplantation at day 7 and month 1. Serum IL-18, creatinine, cystatin C, CRP, and oxidative stress markers (TOS, TAC) were measured. The Oxidative Stress Index (OSI) was calculated. Polymorphisms of the promoter region of the IL-18 gene, IL18-607A/C, and -137C/G were determined by analysis of a real-time PCR/Melting curve. Results Serum creatinine, cystatin C, CRP, IL-18, TOS, and OSI levels significantly decreased after transplantation. Post-transplant levels of serum TAC and estimated GFR demonstrated consistent significant increases. Serum IL-18 levels were significantly higher in patients with IL-18-137 GG and IL-18-607CC genotypes before transplantation. Conclusion Our results indicate that the IL-18-137 GG and -607CC genotypes contribute to higher IL-18 levels; however, the influence of these polymorphisms on oxidative stress has not been observed.