Akademik Veri Yönetim Sistemi
CRISPR and Plant Functional Genomics, Jen‑Tsung Chen, Editör, CRC, New York , London, ss.189-205, 2024
Our understanding of plant–pathogen interactions has undergone significant advancements since the establishment of the gene‑for‑gene theory proposed by Harold Henry Flor [1,2]. Flor’s studies on the genetic interactions between host resistance genes and pathogen avirulence genes paved the way for further investigations in plant pathology. Over time, the field has witnessed remarkable progress with the advent of molecular biology techniques, such as RNA interference (RNAi) and transgene technologies, enabling precise manipulation of organism genomes, including plants. Among the notable developments in molecular biology, the emergence of site‑specific nucleases has played a pivotal role in unraveling the intricacies of plant–pathogen interactions and facilitating the generation of disease‑resistant plants. Particularly, the optimization of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR‑associated protein 9) technology has revolutionized our ability to manipulate the genomes of model organisms as well as plants, offering a rapid and efficient means to generate mutant lines [3,4]. This powerful tool has opened new
avenues for studying the regulatory elements involved in plant–pathogen interactions by creating
loss‑of‑function mutants and expanding our knowledge in this field. In this chapter, we will delve
into the utilization of CRISPR‑based approaches to advance our understanding of plants and their interactions with phytopathogens, drawing upon the wealth of previous research findings. We will explore how CRISPR technology has enabled precise manipulation of host genes, allowing researchers to dissect the complex molecular mechanisms underlying plant defense responses. Furthermore,
we will address the potential of this technology in enhancing plant resistance by precisely modifying host genes and developing commercially viable disease‑resistant plants.