Sustained hypoglycemia affects glucose transporter expression of human blood leukocytes

Korgun E. T., Demir R., Sedlmayr P., Desoye G., Arikan G., Puerstner P., ...Daha Fazla

BLOOD CELLS MOLECULES AND DISEASES, cilt.28, sa.2, ss.152-159, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1006/bcmd.2002.0504
  • Dergi Adı: BLOOD CELLS MOLECULES AND DISEASES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.152-159
  • Anahtar Kelimeler: GLUT granulocyte, leukocytosis, lymphocyte, monocyte, starvation, CIRCULATING MONONUCLEAR-CELLS, TERM PLACENTAL TROPHOBLAST, IN-VITRO, SKELETAL-MUSCLE, MECHANISM, FAMILY, SYSTEM, PHYTOHEMAGGLUTININ, LYMPHOCYTES, HOMEOSTASIS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The scarce data available on leukocyte glucose transporter expression are contradictory and nothing is known about its regulation by glycemic state. Therefore, cytospin preparations of blood leukocytes were searched immunocytochemically for the high-affinity glucose transporters GLUT1, 3, and 4. Hypoglycemia-associated quantitative changes in transporter expression were assessed by flow cytometry. Granulocytes and monocytes stained for GLUT1, 3, and 4. Granulocyte GLUT4 levels were increased by 73% (P < 0.05) under hypoglycemic conditions, which was paralleled by a reduction in GLUT1 and a rise in GLUT3. In monocytes, GLUT3 was elevated by 134% (P < 0.05), whereas GLUT1 and GLUT4 remained unaffected upon hypoglycemia. Apart from a minor subpopulation, lymphocytes were negative for these carriers. In conclusion, GLUT1, 3, and 4 are abundantly expressed in granulocytes and monocytes. The differential response of individual isoforms to hypoglycemia may represent a mechanism to protect the cells from the stress of glucose deprivation. (C) 2002 Elsevier Science (USA).