Analysis of serum cytokine levels in larynx squamous cell carcinoma and dysplasia patients.
Iranian journal of immunology : IJI, vol.11, no.4, pp.259-68, 2014 (SCI-Expanded)
- Publication Type: Article / Article
- Volume: 11 Issue: 4
- Publication Date: 2014
- Journal Name: Iranian journal of immunology : IJI
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.259-68
- Keywords: Bead Array, Cytokine, Dysplasia, HNSCC, Larynxm Carcinoma, NECK-CANCER, BRONCHOPULMONARY DYSPLASIA, HEAD, INTERLEUKIN-6, SURVIVAL, ROLES
- Akdeniz University Affiliated: Yes
Abstract
Background: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation. Objective: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls. Methods: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-alpha (TNF-alpha) and Interferon-gamma (IFN-gamma) levels. Additionally, IL-8 levels were determined through ELISA. Results: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05). Conclusion: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.