Melon Necrotic Spot Virus (MNSV), A Newly Reported Virus Diseases in Squash Species for Turkey

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Koç G., Fidan H., Baloğlu S.

Journal of Turkish Phytopathology, vol.43, no.1, pp.1-7, 2014 (Peer-Reviewed Journal)

Abstract

Abstract
The study was conducted on summer squash (Cucurbita pepo. Linneus, 1753) grown in the plateau of Pozantı
which is a county of Adana, Turkey in 2013. During field surveys, systemic chlorotic rings and irregular
yellowish dark brown necrotic spots were observed on squash plants. For identification, 49 leaf samples were
tested by DAS-ELISA using polyclonal antiserum specific to viruses commonly infecting cucurbits including
Squash mosaic virus (SqMV), Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus(ZYMV),
Watermelon mosaic virus 2(WMV-2), Watermelon mosaic virus 1( WMV-1), Tomato ringspot virus (ToRSV),
Tobacco ringspot virus(TRSV) and Melon necrotic spot virus (MNSV). Three samples were tested positive for
MNSV in DAS-ELISA and the results were confirmed by RT-PCR using total RNA extracted from leaves
(100mg). RT-PCR assay was conducted by Sense MNS-C10 and antisense MNS-N40 primer pairs specific to
MNSV. As a result of RT-PCR, 485 bp cDNA bands were observed in 2% agarose gel. While occurrence of the
ZYMV detected in five samples, remaining viruses were not detected in the tested samples. In Turkey, the
occurrence of MNSV was previously reported at melon field samples by serological technique from Thrace
region in 2006, also it was detected in cucurbit breeding materials by serologically and molecular techniques in
Antalya in 2009. This study is not only first record of MNSV at squash in Turkey but also provide evidence of
uncontrolled distribution among both species and regions.
Key Words: Squash, MNSV, Pozantı, DAS-ELISA, RT-PCR