Distributions of PCNA and Cas-3 in rat uterus during early pregnancy
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA, cilt.48, sa.1, ss.71-77, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 48 Sayı: 1
- Basım Tarihi: 2010
- Doi Numarası: 10.2478/v10042-008-0088-2
- Dergi Adı: FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.71-77
- Anahtar Kelimeler: PCNA, Cas-3, early pregnancy, uterine, EPITHELIAL-CELL DEATH, CHORIOALLANTOIC PLACENTA FORMATION, UTERINE LUMINAL EPITHELIUM, EMBRYO IMPLANTATION, APOPTOSIS, EXPRESSION, DECIDUALIZATION, LOCALIZATION, PATTERNS, INVASION
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Akdeniz Üniversitesi Adresli: Hayır
Özet
The aim of present study was to determine the distributions of proliferating cell nuclear agent (PCNA) and Caspase-3 (Cas-3) and their possible roles in implantation and decidualization during early pregnancy at immunohistochemical level. The tissue samples from pregnant animals between gestational days 1-5 were incubated by PCNA and Cas-3 anti-bodies and the obtained results were evaluated quantitatively. It was observed that PCNA immunoreactivity in uterine luminal epithelium and glandular epithelium reduced as from day 2 of gestation and disappeared as from day 4 of gestation. PCNA staining intensity in stromal cells and myometrium increased gradually with progressing gestation. While Cas-3 immunoreactivity was strongly detected in luminal and glandular epithelium throughout the whole gestational period, its reactivity markedly increased as from day 3 of gestation. In conclusion, it may suggest that the blastocyst implantation induces the uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus.