Distributions of PCNA and Cas-3 in rat uterus during early pregnancy

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ÖNER H., Oner J., Demir R.

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA, vol.48, no.1, pp.71-77, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 1
  • Publication Date: 2010
  • Doi Number: 10.2478/v10042-008-0088-2
  • Journal Name: FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.71-77
  • Keywords: PCNA, Cas-3, early pregnancy, uterine, EPITHELIAL-CELL DEATH, CHORIOALLANTOIC PLACENTA FORMATION, UTERINE LUMINAL EPITHELIUM, EMBRYO IMPLANTATION, APOPTOSIS, EXPRESSION, DECIDUALIZATION, LOCALIZATION, PATTERNS, INVASION
  • Akdeniz University Affiliated: No

Abstract

The aim of present study was to determine the distributions of proliferating cell nuclear agent (PCNA) and Caspase-3 (Cas-3) and their possible roles in implantation and decidualization during early pregnancy at immunohistochemical level. The tissue samples from pregnant animals between gestational days 1-5 were incubated by PCNA and Cas-3 anti-bodies and the obtained results were evaluated quantitatively. It was observed that PCNA immunoreactivity in uterine luminal epithelium and glandular epithelium reduced as from day 2 of gestation and disappeared as from day 4 of gestation. PCNA staining intensity in stromal cells and myometrium increased gradually with progressing gestation. While Cas-3 immunoreactivity was strongly detected in luminal and glandular epithelium throughout the whole gestational period, its reactivity markedly increased as from day 3 of gestation. In conclusion, it may suggest that the blastocyst implantation induces the uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus.