Superovulation alters DNA methyltransferase protein expression in mouse oocytes and early embryos

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Uysal F., ÖZTÜRK S., AKKOYUNLU G.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, cilt.35, sa.3, ss.503-513, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10815-017-1087-z
  • Dergi Adı: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.503-513
  • Anahtar Kelimeler: DNMT, DNA methylation, Early embryo, Oocyte, Superovulation, FOLLICLE-STIMULATING-HORMONE, REPRODUCTIVE TECHNOLOGIES, GENE-EXPRESSION, BIRTH-DEFECTS, MESSENGER-RNA, METHYLATION, PERTURBATIONS, EPIGENETICS, RECEPTORS, DNMT3A
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Purpose DNA methylation is an epigenetic mechanism that plays critical roles during mammalian oocyte and preimplantation embryo development. It is achieved by adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine (CpG) and non-CpG dinucleotide sites using DNA methyltransferase (DNMT) enzymes for de novo and maintenance methylation processes. DNMT1, DNMT3A, and DNMT3B play important roles in establishing methylation of developmentally related genes in oocytes and early embryos. The purpose of this study is to identify the effect of superovulation on the expression and subcellular localizations of these three DNMT enzymes in the mouse oocytes and early embryos.