Evaluation of antimicrobial efficacy of sodium hypochlorite, propolis, octenidine dihydrochloride and chlorhexidine on microorganisms


Kuştarci A., OKTAY E. A., KILIÇ A., ÖZAN U., ALTUNBAŞ D.

Cumhuriyet Dental Journal, cilt.14, sa.3, ss.183-190, 2011 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2011
  • Dergi Adı: Cumhuriyet Dental Journal
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.183-190
  • Anahtar Kelimeler: Chlorhexidine, Microorganisms, Octenidine dihydrochloride, Propolis, Sodium hypochlorite
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

Objectives: The aim of this present study was to evaluate the antimicrobial effect of 2.5% sodium hypochlorite (NaOCl), 12.5% propolis, 25% propolis, octenidine dihydrochloride (OCT) and 2% chlorhexidine (CHX) on microorganisms with different structural characteristics. Materials and Methods: S. Aureus, E. Faecalis, E. Coli and C. Albicans were included in the study. Pre-sterilized paper discs (6 mm in diameter) were soaked with the test solutions and placed on the plates, following Muller- Hinton agar plates were inoculated with the microbial suspensions. Then zones of inhibition were recorded and the results were analysed statistically. 2.5% NaOCl, 2% CHX and OCT produced inhibitory zones against all microorganisms tested. Statistical analysis was carried out with analyses of variance (ANOVA). Differences were identified by post-hoc Bonferroni test. The level of significance was set at p=0.05. Results: NaCl was ineffective against all microorganisms; however, 2.5% NaOCl, 2% CHX and OCT produced inhibitory zones against all microorganisms tested. 2.5% NaOCl and 2% CHX showed significantly larger average zones of inhibition compared to the other experimental irrigants (p<0.05). While 12.5% propolis extract produced only slight inhibition on S. Aureus, 25% propolis extract was effective on S. Aureus, E. Faecalis and C. Albicans. Conclusions: The present study showed that 2.5% NaOCl and 2% chlorhexidine had superior antimicrobial effects than other irrigants used.