MicroRNA-29a plays a prominent role in PRIMA-1(Met)-induced apoptosis in ovarian cancer cells

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İMİR N.

ARCHIVES OF BIOLOGICAL SCIENCES, cilt.72, sa.2, ss.173-179, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.2298/abs191128011i
  • Dergi Adı: ARCHIVES OF BIOLOGICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.173-179
  • Anahtar Kelimeler: apoptosis, miRNA29a, ovarian cancer, PRIMA-1(Met), PRIMA-1 INDUCES APOPTOSIS, MUTANT P53, INHIBITS GROWTH, CARRYING MUTANT, REACTIVATION, PRIMA-1(MET)/APR-246, OVEREXPRESSION, MECHANISMS, MUTATIONS, NETWORK
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The structural analog of the small 2,2-bis(hydroxymethyl)-1-azabicyclo[2,2,2]octan-3-one molecule named PRIMA-1(Met) for "p53 reactivation and induction of massive apoptosis" has been shown to inhibit cell growth and induce apoptosis in human tumor cells by restoring the tumor suppressor function of tumor protein p53. In several microRNA (miRNA) profiling studies related to ovarian cancer, different miRNAs associated with PRIMA-1(Met) have been reported, but miRNAs related to PRIMA-1(Met)-induced apoptosis remain unclear. This study was designed to explain the potential mechanism of PRIMA-1-induced apoptosis. According to the MTSassay and fluorescence-activated cell sorting (FACS) analysis results, PRIMA-1(Met) induced a significant decrease in cell viability and an increase in apoptosis in both A2780 and Caov-3 cells, regardless of p53 status. PRIMA-1(Met) upregulated miRNA-29a in both cell lines. To determine the effect of miRNA-29a on PRIMA-1(Met)-induced apoptosis, A2780 and Caov-3 cells were transfected with miRNA-29a inhibitor. After treatment with PRIMA-1(Met), cell viability increased and apoptosis decreased in the transfected cells. The results of this study suggest that miRNA-29a potentially regulates PRIMA-1(Met)-induced apoptosis in ovarian cancer cells.