N-acetylcysteine enhances multidrug resistance-associated protein 1 mediated doxorubicin resistance
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, vol.34, no.10, pp.683-689, 2004 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 34 Issue: 10
- Publication Date: 2004
- Doi Number: 10.1111/j.1365-2362.2004.01411.x
- Journal Name: EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.683-689
- Keywords: BSO, doxorubicin, GSH, HEK293, MRP1, N-acetylcysteine, ACETYL-L-CYSTEINE, INHIBITS APOPTOSIS, CELL-LINE, MRP GENE, GLUTATHIONE, OVEREXPRESSION, EXPRESSION, SUPPRESSION
- Akdeniz University Affiliated: Yes
Abstract
Background Resistance of cancer cells against anticancer agents is caused partly by multidrug resistance-associated protein 1 (MRP1). The exact mechanism of MRP1-involved multidrug resistance has not yet been clarified, although glutathione (GSH) is likely to have a role for the resistance to occur. N-acetylcysteine (NAC) is a pro-glutathione drug. DL-buthionine (S,R)-sulfoximine (BSO) inhibits GSH synthesis. The aim of our study was to investigate the effect of NAC and BSO on MRP1-mediated doxorubicin resistance in human embryonic kidney (HEK293) and its MRP1-transfected 293MRP cells.