The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes

Yesilkaya A., Yegin A., Ozdem S., Aksu T. A.

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, cilt.28, sa.4, ss.230-234, 1998 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 4
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1007/bf02874114
  • Dergi Adı: INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.230-234
  • Anahtar Kelimeler: lipid peroxidation, bilirubin, antioxidant enzymes, cumene hydroperoxide, erythrocytes, SUPEROXIDE-DISMUTASE, CHEMILUMINESCENCE, PLASMA, BLOOD
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Recently, it has been suggested that bilirubin may act as a potent biological chain-breaking antioxidant. To observe the effects of free bilirubin on antioxidant reactions in cumene hydroperoxide-treated erythrocytes (15 g hemoglobin/dl), we added bilirubin at four different concentrations (0.5, 1, 5, and 10 mg/dl). We measured the thiobarbituric acid-reactive substance and reduced,glutathione levels, and some antioxidant enzyme activities, namely superoxide dismutase, catalase, and glucose-6-phosphate dehydrogenase. Thiobarbituric acid-reactive substance and chemiluminescent signals decreased during the incubation. Superoxide dismutase activities also decreased but not as much as in the control group. Glucose-6-phosphate dehydrogenase activities and reduced glutathione levels increased, but catalase activities remained the same as the control group. Our results suggest that bilirubin - in the concentrations we have used - partially prevented the oxidant effects of cumene hydroperoxide.