Agrobacterium-mediated transformation of Arabis gunnisoniana


TAŞKIN K. M., Turgut K., ERCAN A., SCOTT R.

PLANT CELL TISSUE AND ORGAN CULTURE, vol.72, no.2, pp.173-179, 2003 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1023/a:1022291324492
  • Journal Name: PLANT CELL TISSUE AND ORGAN CULTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.173-179
  • Keywords: Agrobacterium, apomixis, Arabis, in vitro, regeneration, transformation, SOMATIC EMBRYOGENESIS, IMMATURE COTYLEDONS, ARABIDOPSIS-THALIANA, PLANT-REGENERATION, SHOOT REGENERATION, FREQUENCY, FERTILIZATION, ORGANOGENESIS, INHERITANCE, ELIMINATION
  • Akdeniz University Affiliated: Yes

Abstract

An efficient method for adventitious shoot regeneration for Arabis drummondii and a transformation protocol for A. gunnisoniana from hypocotyl explants are described. Hypocotyl explants from 7-day-old aseptically grown seedlings were cultured on MS medium containing plant growth regulators (6-benzylaminopurine, 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea, alpha-naphthaleneacetic acid and 2,4-dichlorophenoxy- acetic acid). After 4 weeks in culture, high frequency of adventitious shoot regeneration was observed. Regenerated shoots were rooted on half-strength MS basal medium supplemented 1% (w/v) sucrose, with or without NAA. This protocol was then used to produce transformed Arabis gunnisoniana plants. A. gunnisoniana hypocotyl explants were co-cultivated with Agrobacterium tumefaciens strain GV3101 harbouring pBJ40. Transgenic shoots were selected on MS medium supplemented with 50 mg l(-1) kanamycin. PCR analysis verified the presence of the nptII gene in the plant DNA isolated from kanamycin resistant shoots.