Effects of abamectin exposure on male fertility in rats: Potential role of oxidative stress-mediated poly(ADP-ribose) polymerase (PARP) activation

Celik-Ozenci C., TAŞATARGİL A., Tekcan M., Sati L., Gungor E., Isbir M., ...Daha Fazla

REGULATORY TOXICOLOGY AND PHARMACOLOGY, cilt.61, sa.3, ss.310-317, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.yrtph.2011.09.001
  • Dergi Adı: REGULATORY TOXICOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.310-317
  • Anahtar Kelimeler: Abamectin, Pesticide, Male infertility, Rat, PARP, NITRIC-OXIDE, SPERM, AVERMECTINS, IVERMECTIN, QUALITY, CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Despite the known adverse effects of abamectin pesticide, little is known about its action on male fertility. To explore the effects of exposure to abamectin on male fertility and its mechanism, low (1 mg/kg/day) and high dose (4 mg/kg/day) abamectin were applied to male rats by oral gavage for 1 week and for 6 weeks. Weight of testes, serum reproductive hormone levels, sperm dynamics and histopathology of testes were used to evaluate the reproductive efficiency of abamectin-exposed rats. Abamectin level was determined at high concentrations in plasma and testicular tissues of male rats exposed to this pesticide. The testes weights of animals and serum testosterone concentrations did not show any significant changes after abamectin exposure. Abamectin administration was associated with decreased sperm count and motility and increased seminiferous tubule damage. In addition, significant elevations in the 4-hydroxy-2-nonenal (4-HNE)-modified proteins and poly(ADP-ribose) (PAR) expression, as markers for oxidative stress and poly(ADP-ribose) polymerase (PARP) activation, were observed in testes of rats exposed to abamectin. These results showed that abamectin exposure induces testicular damage and affects sperm dynamics. Oxidative stress-mediated PARP activation might be one of the possible mechanism(s) underlying testicular damage induced by abamectin. (C) 2011 Elsevier Inc. All rights reserved.

Effects of abamectin exposure on male fertility in rats: Potential role of oxidative stress-mediated poly(ADP-ribose) polymerase (PARP) activation

By:Celik-Ozenci, C (Celik-Ozenci, Ciler)[ 1 ] ; Tasatargil, A (Tasatargil, Arda)[ 2 ] ; Tekcan, M (Tekcan, Merih); Sati, L (Sati, Leyla); Gungor, E (Gungor, Ece); Isbir, M (Isbir, Mehmet)[ 2 ] ; Demir, R (Demir, Ramazan)

 

REGULATORY TOXICOLOGY AND PHARMACOLOGY

 

Volume: 61

Issue: 3

Pages: 310-317

DOI: 10.1016/j.yrtph.2011.09.001

Published: DEC 2011

View Journal Information

REGULATORY TOXICOLOGY AND PHARMACOLOGY

JCR® Category Quartile in Category
MEDICINE, LEGAL Q2
PHARMACOLOGY & PHARMACY Q3
TOXICOLOGY Q3

Data from the 2012 edition of Journal Citation Reports®

Publisher ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA

ISSN: 0273-2300

Research Domain Legal Medicine
Pharmacology & Pharmacy
Toxicology

Abstract

Despite the known adverse effects of abamectin pesticide, little is known about its action on male fertility. To explore the effects of exposure to abamectin on male fertility and its mechanism, low (1 mg/kg/day) and high dose (4 mg/kg/day) abamectin were applied to male rats by oral gavage for 1 week and for 6 weeks. Weight of testes, serum reproductive hormone levels, sperm dynamics and histopathology of testes were used to evaluate the reproductive efficiency of abamectin-exposed rats. Abamectin level was determined at high concentrations in plasma and testicular tissues of male rats exposed to this pesticide. The testes weights of animals and serum testosterone concentrations did not show any significant changes after abamectin exposure. Abamectin administration was associated with decreased sperm count and motility and increased seminiferous tubule damage. In addition, significant elevations in the 4-hydroxy-2-nonenal (4-HNE)-modified proteins and poly(ADP-ribose) (PAR) expression, as markers for oxidative stress and poly(ADP-ribose) polymerase (PARP) activation, were observed in testes of rats exposed to abamectin. These results showed that abamectin exposure induces testicular damage and affects sperm dynamics. Oxidative stress-mediated PARP activation might be one of the possible mechanism(s) underlying testicular damage induced by abamectin. (C) 2011 Elsevier Inc. All rights reserved.