Assessment of axonal sprouting and motor performance after hypoglossal-facial end-to-side nerve repair: experimental study in rats

SARIKCIOĞLU L., HİZAY A., ÖZSOY U., Kandemir Y. B., ŞEKERCİ R., Angelov D. N.

EXPERIMENTAL BRAIN RESEARCH, cilt.238, sa.6, ss.1563-1576, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 238 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s00221-020-05835-6
  • Dergi Adı: EXPERIMENTAL BRAIN RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Psycinfo, Veterinary Science Database
  • Sayfa Sayıları: ss.1563-1576
  • Anahtar Kelimeler: Facial nerve, Hypoglossal nerve, End-to-side coaptation, Functional recovery, HFA, LONG-TERM EVALUATION, Y-TUBE CONDUIT, PERIPHERAL-NERVE, MANUAL STIMULATION, TERMINOLATERAL NEURORRHAPHY, MORPHOMETRIC EVALUATION, NEURON REGENERATION, SCHWANN-CELLS, SCIATIC-NERVE, GROWTH-FACTOR
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Hypoglossal-facial nerve anastomosis (HFA) aims to reanimate denervated mimic muscles with hypoglossal axons when the transected facial nerve is not accessible. The aim of this study was to evaluate the recovery of HFA using a "Y" tube in two variants: (1) the proximal stump of the hypoglossal nerve was entubulated to the "Y" tube (classic "Y" tube HFA) and (2) the "Y" tube was sutured to an epineurial window of a slightly damaged hypoglossal nerve (end-to-side "Y" tube HFA). A total of 48 adult female rats were divided into four groups: intact controls (group 1), sham operated (group 2), classic "Y" tube HFA (group 3) and end-to-side "Y" tube HFA (group 4). The abdominal aorta with both common iliac arteries of isogeneic male rats served as the Y-tube conduit. Animals from group 4 recovered better than those from group 3: the degree of collateral axonal branching (3 +/- 1%) was significantly lower than that determined in group 3 (13 +/- 1%). The mean deviation of the tongue from the midline was significantly smaller in group 4 (6 +/- 4 degrees) than that measured in animals from group 3 (41 +/- 6 degrees). In the determination of vibrissal motor function in group 3 and group 4, a decrease in amplitude was found to be - 66% and - 92%, respectively. No differences in the reinnervation pattern of the target muscles were detected. As a result, these surgical models were not determined to be able to improve vibrissal movements. It was concluded that performance of end-to-side "Y" tube HFA diminishes collateral axonal branching at the lesion site, which in turn, promotes better recovery of tongue- and vibrissal-motor performance.