Novel enzymatic graphene oxide based biosensor for the detection of glutathione in biological body fluids

Cheraghi S., Taher M. A., Karimi-Maleh H., Karimi F., Shabani-Nooshabadi M., Alizadeh M., ...Daha Fazla

Chemosphere, cilt.287, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 287
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.chemosphere.2021.132187
  • Dergi Adı: Chemosphere
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, MEDLINE, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Enzymatic biosensor, Glutathione, Glutathione peroxidase, Graphene oxide, Nafion, Voltammetric method
  • Akdeniz Üniversitesi Adresli: Evet

Özet

© 2021In this work, we report a novel enzymatic biosensor based on glutathione peroxidase (GSH-Px), graphene oxide (GO) and nafion for the electrochemical sensing of glutathione (GSH) in body fluids. GSH-Px was immobilized covalently via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) onto modified glassy carbon electrode (GCE) decorated with GO and nafion and successfully used for sensing of GSH in the presence of H2O2 as catalyst with Michaelis-Menten constant about 0.131 mmol/L. The active surface are of GCE improve from 0.183 cm2 to 0.225 cm2 after modification with GO. The introduced biosensor (GSH-Px/GO/nafion/GCE) was used for monitoring of GSH over the range 0.003–370.0 μM, with a detection limit of 1.5 nM using differential pulse voltammetric (DPV) method. The GSH-Px/GO/nafion/GCE was successfully applied to the determination of GSH in real samples.