Atıf İçin Kopyala
Şahin Z., Celik-Ozenci C., Akkoyunlu G., Korgun E. T., Acar N., Erdoğru T., ...Daha Fazla
FERTILITY AND STERILITY, cilt.85, ss.1265-1275, 2006 (SCI-Expanded)
-
Yayın Türü:
Makale / Tam Makale
-
Cilt numarası:
85
-
Basım Tarihi:
2006
-
Doi Numarası:
10.1016/j.fertnstert.2005.10.025
-
Dergi Adı:
FERTILITY AND STERILITY
-
Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
-
Sayfa Sayıları:
ss.1265-1275
-
Anahtar Kelimeler:
IL-1 alpha, IL-1 beta, experimental varicocele, rat, testis, RAT LEYDIG-CELLS, CYTOKINES, LIPOPOLYSACCHARIDE, STEROIDOGENESIS, TESTIS
-
Akdeniz Üniversitesi Adresli:
Evet
Özet
Objective: To describe the effect of varicocele, in an experimental rat model, on the levels of IL-1α and IL-1β proteins in testis tissue.
Design: Comparative and controlled study.
Setting: Experimental research.
Animal(s): Wistar male rats in experimental and control groups.
Intervention(s): The control group underwent sham operation (n = 6). Experimental groups underwent partial ligation of the renal vein to induce experimental varicocele and were then killed at 9 (n = 6), 11 (n = 6), and 13 (n = 6) weeks after induction of varicocele.
Main Outcome Measure(s): Histologic evaluation of the varicocele model was determined by periodic acid-Schiff staining of paraffin-embeded testicular tissues. Levels of cytokines were assessed by immunohistochemistry and Western blot analysis.
Result(s): Varicocele caused testicular damage, especially in 11- and 13-week-old varicocele groups. In sham-operated rats, Golgi complexes of round spermatids expressed especially the α form of IL-1. By the progression of varicocele, the IL-1α expression increased temporally in Sertoli cells, spermatogonia, primary spermatocytes, spermatids, and Leydig cells. The expression of IL-1α was seen in Leydig cells in sham-operated rats. The IL-1β expression was also increased upon progression of varicocele in Leydig cells, Sertoli cells, and spermatogonia.
Conclusion(s): We suggest that IL-1α and IL-1β are the regulators of testicular function. Certain pathologic conditions, e.g., varicocele, cause an increase in the expressions of such proinflammatory cytokines. The increased expression of IL-1α and IL-1β in varicocele shifts the balance in favor of inflammatory and immune responses and causes detrimental effects in testis tissue, which may cause male infertility.