Identification of the Genes that Confer Genetic Resistance to Marek's Disease in Chicken Using SNP Markers

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MEYDAN H., YILDIZ M. A., Cheng H. H.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.21, sa.4, ss.449-458, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1501/tarimbil_0000001348
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.449-458
  • Anahtar Kelimeler: ENO1, TPT1, RBBP4, ASE, Pyrosequencing, Genetic resistance, ALLELE-SPECIFIC EXPRESSION, B-HAPLOTYPE INFLUENCE, AFFECTING SUSCEPTIBILITY, RELATIVE EFFICACY, VIRUS-INFECTION, PROTEIN, BROILER
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

Marek's disease (MD), one of the most threatening diseases to poultry industry, is contagious disease of domestic chickens induced by the Marek's disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus. Enhancing resistance to MD in poultry is an attractive method to augment MD vaccines. Because the inadequacy in immune system occurs as a result of vaccine, the other method for avoiding from MD is to breed the chickens that are resistant to MD. In this study, ENO1, TPT1 and RBBP4 genes in chickens were screened whether they played a role in resistance to MD by using allele-specific expression (ASE). The F-1 progeny of reciprocal intermatings between highly inbred chicken line 6 (MD resistant) and line 7 (MD susceptible) were sampled for this study. RNA was isolated from three organ tissues (Bursa fabricius, thymus and spleen) and the relative expression levels of each allele were monitored by using pyrosequencing method. The results suggested that TPT1 and RBBP4 genes have the cis-acting regulatory elements but these cis-acting elements do not play any role in conferring genetic resistance to MD.

Marek’s disease (MD), one of the most threatening diseases to poultry industry, is contagious disease of domestic
chickens induced by the Marek’s disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus.
Enhancing resistance to MD in poultry is an attractive method to augment MD vaccines. Because the inadequacy in
immune system occurs as a result of vaccine, the other method for avoiding from MD is to breed the chickens that are
resistant to MD. In this study, ENO1, TPT1 and RBBP4 genes in chickens were screened whether they played a role in resistance to MD by using allele-specific expression (ASE). The F1 progeny of reciprocal intermatings between highly
inbred chicken line 6 (MD resistant) and line 7 (MD susceptible) were sampled for this study. RNA was isolated from
three organ tissues (Bursa fabricius, thymus and spleen) and the relative expression levels of each allele were monitored
by using pyrosequencing method. The results suggested that TPT1 and RBBP4 genes have the cis-acting regulatory
elements but these cis-acting elements do not play any role in conferring genetic resistance to MD.
Keywords: ENO1; TPT1; RBBP4; ASE; Pyrosequencing; Genetic resistance