Prion protein gene polymorphism and genetic risk evaluation for scrapie in all Turkish native sheep breeds

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Meydan H., YÜCEER B., DEGIRMENCI R., ÖZKAN M., YILDIZ M. A.

VIRUS GENES, cilt.45, sa.1, ss.169-175, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s11262-012-0744-7
  • Dergi Adı: VIRUS GENES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.169-175
  • Anahtar Kelimeler: Scrapie, PrP gene, Polymorphism, Genetic risk, Turkish native sheep, PRP GENE, NATURAL SCRAPIE, HEALTHY, FREQUENCIES, GENOTYPE
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

The aim of this study was to identify the prion protein (PrP) gene polymorphism in a total of 1,110 healthy sheep from 18 Turkish native sheep breeds. There were nine alleles and 22 genotypes observed based on codons 136, 154, and 171 of the PrP gene. The ARQ allele was predominant for all breeds. The most resistant allele to scrapie, ARR, was present in all breeds. The VRQ allele, associated with the highest susceptibility to scrapie, was detected at low frequencies in A degrees vesi (0.06), KA +/- vA +/- rcA +/- k (0.021), SakA +/- z (0.010), Karayaka (0.011), Cine CaparA +/- (0.012), and Guneykaraman (0.017). In general, the ARQ/ARQ genotype was predominant in all breeds. The most resistant genotype to scrapie, ARR/ARR, was found with the frequency lower than 0.180. The most susceptible genotype, VRQ/VRQ, was found in only KA +/- vA +/- rcA +/- k. The TRR and TRH alleles and the genotypes of ARR/TRR, ARR/ARK, and ARH/TRH have been found for the first time in Turkish native sheep breeds. According to these results, all breeds belong to risk group R3 followed by R2. It is propounded that the susceptibility to scrapie increased from eastern to western part of Turkey. Our findings of Turkish native sheep breeds with PrP gene polymorphisms will assist the sheep breeding program for selection of scrapie resistance genotypes to reduce the risk of scrapie.

Abstract The aim of this study was to identify the prion
protein (PrP) gene polymorphism in a total of 1,110 healthy
sheep from 18 Turkish native sheep breeds. There were
nine alleles and 22 genotypes observed based on codons
136, 154, and 171 of the PrP gene. The ARQ allele was
predominant for all breeds. The most resistant allele to
scrapie, ARR, was present in all breeds. The VRQ allele,
associated with the highest susceptibility to scrapie, was
detected at low frequencies in I'vesi (0.06), Kıvırcık
(0.021), Sakız (0.010), Karayaka (0.011), C¸ ine C¸ aparı
(0.012), and Gu¨neykaraman (0.017). In general, the ARQ/
ARQ genotype was predominant in all breeds. The most
resistant genotype to scrapie, ARR/ARR, was found with
the frequency lower than 0.180. The most susceptible
genotype, VRQ/VRQ, was found in only Kıvırcık. The
TRR and TRH alleles and the genotypes of ARR/TRR,
ARR/ARK, and ARH/TRH have been found for the first
time in Turkish native sheep breeds. According to these
results, all breeds belong to risk group R3 followed by R2.
It is propounded that the susceptibility to scrapie increased
from eastern to western part of Turkey. Our findings of
Turkish native sheep breeds with PrP gene polymorphisms
will assist the sheep breeding program for selection of
scrapie resistance genotypes to reduce the risk of scrapie.


Keywords Scrapie  PrP gene  Polymorphism  Genetic
risk  Turkish native sheep