alpha-Actinin Anchors PSD-95 at Postsynaptic Sites
NEURON, vol.97, no.5, pp.1094-1118, 2018 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 97 Issue: 5
- Publication Date: 2018
- Doi Number: 10.1016/j.neuron.2018.01.036
- Journal Name: NEURON
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.1094-1118
- Open Archive Collection: AVESIS Open Access Collection
- Akdeniz University Affiliated: Yes
Abstract
Despite the central role PSD-95 plays in anchoring postsynaptic AMPARs, how PSD-95 itself is tethered to postsynaptic sites is not well understood. Here we show that the F-actin binding protein alpha-actinin binds to the very N terminus of PSD-95. Knockdown (KD) of alpha-actinin phenocopies KD of PSD-95. Mutating lysine at position 10 or lysine at position 11 of PSD-95 to glutamate, or glutamate at position 53 or glutamate and aspartate at positions 213 and 217 of alpha-actinin, respectively, to lysine impairs, in parallel, PSD-95 binding to alpha-actinin and postsynaptic localization of PSD-95 and AMPARs. These experiments identify alpha-actinin as a critical PSD-95 anchor tethering the AMPAR-PSD-95 complex to postsynaptic sites.