Influences of different stress models on the antioxidant status and lipid peroxidation in rat erythrocytes
FREE RADICAL RESEARCH, cilt.36, sa.12, ss.1277-1282, 2002 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 12
- Basım Tarihi: 2002
- Doi Numarası: 10.1080/1071576021000016508
- Dergi Adı: FREE RADICAL RESEARCH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1277-1282
- Anahtar Kelimeler: cold stress, immobilization stress, cold plus immobilization stress, antioxidant enzymes, lipid peroxidation, erythrocyte, rat, BROWN ADIPOSE-TISSUE, GLUTATHIONE METABOLISM, IMMOBILIZATION STRESS, ENZYME-ACTIVITIES, OXIDATIVE DAMAGE, COLD, PROTECTION, BRAIN, AUTOXIDATION, ACCLIMATION
- Akdeniz Üniversitesi Adresli: Evet
Özet
The aim of this study was to investigate the influences of different stress models on the antioxidant status and lipid peroxidation (LPO) in erythrocytes of rats. Swiss-Albino female rats (3 months old) were used in this study. Rats were randomly divided into the following four groups; control group (C), cold stress group (CS), immobilization stress group (IS) and cold+immobilization stress group (CS+IS). Control group was kept in an animal laboratory (22+/-2degreesC). Rats in CS group were placed in cold room (5degreesC) for 15 min/day for 15 days. Rats in IS group were immobilized for 180 min/day for 15 days. Rats in CS+IS group were exposed to both cold and immobilization stresses for 15 days. At the end of experimental periods, the activities of glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and concentration of reduced glutathione (GSH) were measured. LPO was determined by measuring the contents of thiobarbituric acid-reactive substances (TBARS). Cu,Zn-SOD activity and TBARS concentration were increased after cold and immobilization stresses, but CAT and GSH-Px activities and GSH levels were decreased. Immobilization stress decreased the activity of G-6-PD. The activities of G-6-PD, CAT and GSH-Px, and the level of GSH were lower in CS+IS group than in the control group. Cu,Zn-SOD activity and TBARS levels were increased in CS+IS group when compared with the control group. From these findings, three stress models are thought to cause oxidative stress.