IN VITRO CLONAL PROPAGATION OF ENDEMIC ALLIUM JUNCEUM SUBS. TRIDENTATUM VIA IMMATURE AND MATURE EMBRYO CULTURE METHOD

NASIRCILAR A. G.

COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES, vol.74, no.8, pp.1256-1266, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 8
  • Publication Date: 2021
  • Doi Number: 10.7546/crabs.2021.08.18
  • Journal Name: COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, zbMATH
  • Page Numbers: pp.1256-1266
  • Keywords: Allium junceum, embryo culture, endemic, ornamental, somatic embryogenesis, PLANT-REGENERATION, SOMATIC EMBRYOGENESIS, EXPLANTS, GROWTH
  • Akdeniz University Affiliated: Yes

Abstract

Allium junceum subs. tridentatum is an endemic bulbous plant that has the potential to be used as an ornamental plant and requires conservation in wild populations. The effects of various plant growth regulators on the regeneration capacity of immature and mature embryos were investigated in vitro. Embryos of mature and immature seeds were propagated by tissue culture; callus, shoot and bulb formation percentages (%), shoot and bulb formation per explant (number), callus weight (g) and shoot length (mm) were determined. Although mature embryos gave better results in terms of shoot and bulb formation percentage (100%, 90%, respectively, on MS media containing 1 mg/L BAP + 0.5 mg/L IAA), immature embryos formed more shoots (8.53) and bulbs (3.33) per explant than mature embryos on MS medium containing 4 mg/L BAP + 1 mg/L IAA. In this study, plants ready to be transferred to soil were obtained in quite a short time and it is thought that the method could be used to produce other commercial Allium species.