Effect of orexin-A on phagocytic activity of peritoneal macrophage in starved rats

Izgut-Uysal V. N., Gemici B., Tan R.

CELLULAR IMMUNOLOGY, cilt.271, sa.1, ss.85-88, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 271 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.cellimm.2011.06.005
  • Dergi Adı: CELLULAR IMMUNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.85-88
  • Anahtar Kelimeler: Phagocytic activity, Macrophages, Orexin-A, SB-334867, Fasting, ISCHEMIA-REPERFUSION INJURY, TOLL-LIKE RECEPTORS, NITRIC-OXIDE, OXIDATIVE STRESS, ACUTE STARVATION, LEPTIN, INFLAMMATION, GLUCOSE, BRAIN, BLOOD
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the effect of fasting-induced orexin-A (OXA) on inflammation and macrophage phagocytic activity. Fifty six male wistar rats were fasted for 36 h to stimulate OXA synthesis. In 24 rats, air pouches were induced subcutaneously in the intrascapular area. After (6 h) carrageenan injection into the pouches, the contents of the air pouches were removed. The exudate volume, protein content and cell count were measured. After the determination of fasting on inflammation, the peritoneal macrophages were collected from 32 rats to investigate the effect of fasting-induced OXA on macrophage phagocytic activity. Plasma OXA levels were markedly higher in fasted rats compared with control rats. The phagocytic capability of peritoneal macrophages was obtained as a percentage of phagocytosing macrophages and number of phagocytosed particles per cell. In spite of increased blood OXA level SB-334867, selective orexin type 1 receptor antagonist (10 mg/kg) did not change phagocytic activity of peritoneal macrophages. These findings indicate that 36 h fasting-induced OXA has no significant effect to phagocytosis of peritoneal macrophages. (C) 2011 Elsevier Inc. All rights reserved.