Construction of ferrocene modified conducting polymer based amperometric urea biosensor

Dervisevic M., Dervisevic E., Senel M., Cevik E., YILDIZ H. B., ÇAMURLU P.

ENZYME AND MICROBIAL TECHNOLOGY, cilt.102, ss.53-59, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 102
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.enzmictec.2017.04.002
  • Dergi Adı: ENZYME AND MICROBIAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.53-59
  • Anahtar Kelimeler: Urea, Urease, Amperometric biosensor, SNS aniline, Ferrocene, COMPOSITE ELECTRODE, FLOW-INJECTION, SENSOR, FILM, CHROMATOGRAPHY, NANOPARTICLES, POLYPYRROLE, NITROGEN, SERUM
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor's amperometric response was linear in the range of 0.1-8.5 mM with the sensitivity of 0.54 mu A/mM, detection limit of 12 mu M, and short response time of 2 s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insignificant interference.