Immune modulatory and antioxidant effects of locally administrated vitamin C in experimental periodontitis in rats


AYTEKİN Z., ARABACI T., Toraman A., BAYIR Y., ALBAYRAK M., ÜSTÜN K.

ACTA ODONTOLOGICA SCANDINAVICA, cilt.78, sa.6, ss.425-432, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 78 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/00016357.2020.1734656
  • Dergi Adı: ACTA ODONTOLOGICA SCANDINAVICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Artic & Antarctic Regions, BIOSIS, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.425-432
  • Anahtar Kelimeler: Experimental periodontitis, oxidative stress, antioxidant, vitamin C, LIGATURE-INDUCED PERIODONTITIS, ALVEOLAR BONE-RESORPTION, ASCORBIC-ACID, OXIDATIVE STRESS, ACHILLES-TENDON, REACTIVE OXYGEN, SERUM, TISSUE, MELATONIN, INTERLEUKIN-1-BETA
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Vitamin C is an important water-soluble vitamin with antioxidant and immune-modulatory actions. The aim of this study was to investigate the effects of locally applied vitamin C on alveolar bone resorption in rats with experimental periodontitis. Methods: Twenty-one male Sprague-Dawley rats divided into three groups with seven animals in each group: (1) control, (2) experimental periodontitis and 3) experimental periodontitis-local vitamin C treatment group. After ligature was removed, 50 mu L vitamin C was locally administered into the subperiosteum of the buccal gingiva of periodontitis vitamin C (PvitC) group rats for three times in intervals of 2 days. At the end of the study, the animals were scarified, and serum and gingival samples were collected for analysis of serum IL-1 beta, oxidative stress index (OSI), CTX and malondialdehyde (MDA) levels and gingival MMP-8 immunostaining. Alveolar bone loss and attachment loss were determined based on measurements on histological sections obtained from rat mandibles. Results: Serum MDA and OSI levels which are related to the oxidative stress were significantly lower in the PvitC group as compared with those in the P group (p < .05). Serum CTX levels which are related to the bone resorption were significantly lower in the PvitC group as compared with those in the P group (p < .05). The numeric density of MMP-8-positive cells was significantly lower in the PvitC group compared to P group (p < .05). Alveolar bone loss and attachment loss were significantly lower in the PvitC group compared to P group (p < .05) Conclusions: The local vitamin C administration provided protection against inflammation-induced alveolar bone resorption by decreasing oxidative stress and inflammation-induced tissue breakdown vitamin C may be a therapeutic agent that can be used in periodontitis treatment.