Research Information System
43. FEBS kongresi, Prag, Czech Republic, 7 - 12 July 2018, pp.1
Bacground/Aim A healthy pregnancy is closely associated with normal placental development. Inadequate placental development may cause clinical complications such as intrauterine growth retardation, preeclampsia and diabetes. In order to prevent the adverse pregnancy outcomes, it is important to understand the hormonal changes and altered glucose metabolism that are associated with diabetes mellitus during pregnancy. Adiponectin, an adipokine, regulates glucose and lipid metabolism and its concentration is negatively correlated with diabetes. This study aims to evaluate the role of adiponectin on placental development in diabetic conditions. Methods This study comprises diabetic and control groups. The control group underwent injection of saline. Diabetes was provided by injection of streptozotocin (STZ, 50 mg/ kg). Female rats whose blood glucose value is higher than 200 mg/dL were considered as diabetic. Adiponectin, its receptors (AdipoR1 and AdipoR2), and its downstream molecules (PPARα, PPARγ) were detected in the control and STZinduced rat placentas on day 14, 16, 18, and 20 of pregnancy by using Western Blot and RTPCR techniques. Serum concentrations of adiponectin were assessed by ELISA method. Results We showed that there is an inversely association between adiponectin, PPARs and diabetes. In the present study, we observed that adiponectin and its receptors levels reduced in diabetic conditions. Furthermore, maternal serum adiponectin levels reduced during the pregnancy in diabetic rats. We also showed that the levels of PPARα, PPARγ and RXRα expression were lower in diabetic rats compare to control groups. Conclusions Based upon those observations above, we believe that adiponectin has a significant role in placental development. In conclusion, pregnancy and diabetes is associated with adiponectin levels.