Secondary metabolites from Scutellaria brevibracteata subsp. subvelutina and their in vitro anti-inflammatory activities

Erdogan M., Konya R., Ozhan Y., SİPAHİ H., ÇİNBİLGEL İ., Masullo M., ...Daha Fazla

SOUTH AFRICAN JOURNAL OF BOTANY, cilt.139, ss.12-18, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 139
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.sajb.2021.01.028
  • Dergi Adı: SOUTH AFRICAN JOURNAL OF BOTANY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.12-18
  • Anahtar Kelimeler: Scutellaria brevibracteata subsp. subvelutina, Lamiaceae, Iridoids, Flavonoids, Anti-inflammatory activity, Nitrite oxide, INDUCED CYCLOOXYGENASE-2 EXPRESSION, NITRIC-OXIDE SYNTHASE, IRIDOID GLYCOSIDES, PLANT FLAVONE, ANTIOXIDANT ACTIVITIES, SKIN INFLAMMATION, MEDICINAL-PLANTS, RECH. F, INHIBITION, GLUCOSIDES
  • Akdeniz Üniversitesi Adresli: Evet

Özet

From the MeOH extract of the aerial parts of Scutellaria brevibracteata subsp. subvelutina 12 secondary metabolites including eight iridoid glycosides (1-8), three flavonoids (9-11) and one phenylethanoid glycoside (12) were isolated. Their structures were elucidated as catalpol (1), globularin (2), scutellarioside II (3), picroside III (4), pensteminoside (5), 8-epiloganic acid (6), albidoside (7), agnucastoside B (8), hispidulin (9), luteolin 7-O-glucuronide (10), hispidulin 7-O-glucuronide (11) and martynoside (12) by using extensive NMR and HR-MS analysis. All compounds are being reported for the first time from S. brevibracteata subsp. subvelutina while compound 5 is new for Lamiaceae family. The chemotaxonomic importance of the isolates was discussed in the detail. All the isolates were evaluated for their in vitro anti-inflammatory activities via their inhibitory actions on the release of nitrite oxide and IL-6 from LPS-induced RAW 264.7 macrophage cells. Compounds 9 and 11 exhibited significant NO reducing capacity at 100 mu M concentration comparable to the positive controls, indomethacin and L-NAME. Moreover, 2, 9 and 10 significantly suppressed the release of IL-6 at the same concentration better than the positive controls. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.