This research expected to determine new durum wheat germplasm resistant to biotic and abiotic stress factors. Eighty durum wheat lines selected from eighteen diverse landraces were tested together with eight durum wheat cultivars under reliable yellow rust epidemic during two successive years. Average infection coefficient of populations and cultivars was 32.44 in 2003 and 26.24 in 2004, showing severe epidemic condition which occurred at adult plant stage in 2003. Because of this the number of selected resistant and moderately resistant plant material greatly reduced. According to the yield trial study in which twenty resistant lines selected out of 30 resistant and moderately from sixteen populations were included, only two checks outperformed grand mean (2.48 t ha (-1)) and two lines selected from landrace population followed these with slightly lower yield difference. On the other hand, there were several lines which performed better than the grand mean of protein content (13.24%), SDS sedimentation (28.40 ml) and semolina color (24.35) and they ranked in the first group including the two checks cultivars. Bi-plot analysis showed that some promising lines with reasonable grain yields, good quality parameters, winter hardiness and drought tolerances among yellow rust resistance durum wheat landraces can be selected for semiarid conditions of Mediterranean countries for sustainable production.