Comparison of promidium monoazide and Reagent D for discrimination of viableAphelenchoides besseyiusing real-time PCR

SERT ÇELİK E., OZALP T., DEVRAN Z.

EUROPEAN JOURNAL OF PLANT PATHOLOGY, vol.158, no.3, pp.767-771, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 158 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.1007/s10658-020-02090-x
  • Journal Name: EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Environment Index, Geobase, Veterinary Science Database
  • Page Numbers: pp.767-771
  • Keywords: Aphelenchoides besseyi, Viability, Propidium monoazide, Reagent D, TaqMan probe, PROPIDIUM MONOAZIDE, APHELENCHOIDES-BESSEYI, LISTERIA-MONOCYTOGENES, QUANTIFICATION, COLI
  • Akdeniz University Affiliated: Yes

Abstract

Aphelenchoides besseyiChristie, 1942 is a seed-borne nematode and causes economic yield losses in rice. Information on the viability ofA. besseyiin stored rice seeds is important to manage this nematode. In this study, propidium monoazide (PMA) and Reagent D, combined with qPCR, were used for discrimination of viableA. besseyiindividuals.A. besseyiindividuals that were live, stored for 1 year or heat-treated (40 or 60 degrees C, 15 min) were exposed to PMA and Reagent-D dyes. In addition,A. besseyiindividuals not treated with any chemicals were used as a control. DNA was then isolated from all individuals and real-time PCR was carried out. Ct values for samples treated with Reagent-D and PMA were different from untreated samples. Results showed that live individuals of the rice white tipe nematode could be distinguished from non-viable individuals using Reagent-D or PMA combined with qPCR.