Investigation of protective effects of selenium and vitamin e against DNA oxidation, membrane damage and alteration of COMT metabolism in smoke-exposed male mice (Mus musculus, Balb/c)
ACTA BIOLOGICA HUNGARICA, vol.57, no.4, pp.403-413, 2006 (SCI-Expanded)
- Publication Type: Article / Article
- Volume: 57 Issue: 4
- Publication Date: 2006
- Doi Number: 10.1556/abiol.57.2006.4.2
- Journal Name: ACTA BIOLOGICA HUNGARICA
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.403-413
- Keywords: mice (Balb/c), vitamin E, selenium, malondialdehyde (MDA), catechol-o-methyl transferase (COMT), 8-hydroxy deoxyguanozine (8-OHdG), CATECHOL-O-METHYLTRANSFERASE, CIGARETTE-SMOKE, LIPID-PEROXIDATION, BIOMARKER EVIDENCE, TOBACCO-SMOKE, 8-HYDROXY-2-DEOXYGUANOSINE, ANTIOXIDANTS, EPIRUBICIN, EXCRETION, TOXICITY
- Akdeniz University Affiliated: Yes
Abstract
In our study, the protective effects of vitamin E and Se ( selenium) against cigarette smoke hazards on second-hand smoker ( passive smoker) male mice ( Balb/c) were investigated. Serum MDA levels in the smoke-exposed mice were found higher than serum MDA levels of control mice and Se-and vitamin E-treated mice. But, the MDA levels of smoke- exposed plus Se- and vitamin E-treated mice were found lower than MDA levels of smoke- exposed mice at the end of the three and five months. According to these results, application of vitamin E and Se, when given to smoke- exposed mice together, had an additive protective effect against cigarette smoke hazards ( p < 0.05). Vitamin E also had protective effect on formation of 8-OHdG in smoke-exposed mice. The serum 8-OHdG amounts of smoke- exposed plus vitamin E-treated mice were found low, but the serum 8-OHdG amounts of smoke- exposed mice were found high. Also 8-OHdG levels in the serum of the smoke- exposed mice were increased which occurs as a result of DNA oxidation ( p < 0.05). At the end of the three and five months, COMT ( catechol-omethyl transferase) activity of smoke- exposed mice livers were increased but, vitamin E and/or Se showed a significant protective effect on changing of COMT activity only at the end of the 5 months. Our results showed that MDA levels and 8-OHdG amounts were increased in the serum of smoke-exposed mice. On the other hand, vitamin E and Se had an additive protective effect against increasing MDA level. Also vitamin E had a protective effect against formation of 8-OHdG amounts and COMT activity alterations.