Changes in glucose-6-phosphate dehydrogenase, copper, zinc-superoxide dismutase and catalase activities, glutathione and its metabolizing enzymes, and lipid peroxidation in rat erythrocytes with age

Ozturk O., GUMUSLU S.

EXPERIMENTAL GERONTOLOGY, vol.39, no.2, pp.211-216, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 2
  • Publication Date: 2004
  • Doi Number: 10.1016/j.exger.2003.10.015
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.211-216
  • Keywords: glucose-6-phosphate dehydrogenase, copper, zinc-superoxide dismutase, selenium-dependent glutathione peroxidase, glutathione-S-transferase, age, glutathione, redox index, lipid peroxidation, erythrocyte, rat, ANTIOXIDANT ENZYMES, OXIDATIVE DAMAGE, S-TRANSFERASE, AGING RAT, CELL, AUTOXIDATION, EXPRESSION, DISULFIDE, SELENIUM, SYSTEMS
  • Akdeniz University Affiliated: Yes


We have studied the activities of enzymes (glucose-6-phosphate dehydrogenase (G-6-PD), copper, zinc-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GSH-Px) and glutathione-S-transferase (GST)), and the levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and thiobarbituric acid-reactive substances (TBARS) in rat erythrocytes and estimated the ratio of GSH/GSSG and the redox index. Male Wistar rats at ages of 1, 6 and 12 months were used. The activities of G-6-PD and Cu,Zn-SOD, the levels of GSSG and TBARS were increased, while the activity of Se-GSH-Px and the level of GSH were decreased with age. GSH/GSSG ratio was significantly decreased with age. We found a positive correlation between age and G-6-PD (r = 0.476, p < 0.0 1), Cu,Zn-SOD (r = 0.291, p < 0.01) CAT (r = 0.254, p < 0.01) and GST activities (r = 0.250, p < 0.05), and GSSG (r = 0.708, p < 0.05) and TBARS levels (r = 0.801 p < 0.01), whereas the correlation between age and Se-GSH-Px activity (r = -0.376, p < 0.05), GSH level (r = -0.603, p < 0.01) and GSH/GSSG ratio (r = -0.685, p < 0.05) were negative. We found age-related differences in erythrocyte antioxidant enzyme activities, GSH, GSSG, total GSH and TBARS levels, GSH/GSSG ratio and the redox index. (C) 2003 Elsevier Inc. All rights reserved.