Surfactant Proteins A and D in the Seminal Plasma of Stallions


ATA A., KANKAVİ O., Ciftcioglu M. A.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.32, no.5, pp.353-358, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 5
  • Publication Date: 2008
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.353-358
  • Keywords: Surfactant protein A (SP-A), surfactant protein D (SP-D), western blotting, stallion, immunohistochemistry, seminal plasma, accessory sex glands, SP-A, REPRODUCTIVE-TRACT, INNATE IMMUNITY, LUNG, COLLECTINS, GENE, EXPRESSION, BINDING, TISSUES, CDNA
  • Akdeniz University Affiliated: Yes

Abstract

We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion's genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP- A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.