The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence

Gumuslu S., Yucel G., Aydin M., Yesilkaya A., Demir A., Aksu T.

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, cilt.26, sa.2, ss.119-123, 1996 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 2
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1007/bf02592354
  • Dergi Adı: INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.119-123
  • Anahtar Kelimeler: chemiluminescence, t-butyl hydroperoxide, ascorbic acid, glutathione, uric acid, taurine, HUMAN-BLOOD PLASMA, ASCORBATE, ERYTHROCYTES, GENERATION, DEFENSE, OXYGEN
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence.

Gümüşlü S1, Yücel G, Aydin M, Yeşilkaya A, Demir AY, Aksu TA.

 

 

Abstract

An experimental system which assesses the antioxidant potential of ascorbic acid, glutathione, uric acid, and taurine was developed. The system comprised hemoglobin, luminol, t-butyl hydroperoxide, and different concentrations of antioxidants in TRIS-HCl buffer (pH 7.4). Control assays were performed by excluding antioxidants. Chemiluminescence was detected using a liquid scintillation counter in single photon mode. All antioxidants, when applied in the appropriate concentrations, decreased the maximum chemiluminescence values. The minimum concentrations which decreased the chemiluminescence values were defined for each of the antioxidants.