The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence

Gumuslu S., Yucel G., Aydin M., Yesilkaya A., Demir A., Aksu T.

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, vol.26, no.2, pp.119-123, 1996 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 26 Issue: 2
  • Publication Date: 1996
  • Doi Number: 10.1007/bf02592354
  • Journal Name: INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.119-123
  • Keywords: chemiluminescence, t-butyl hydroperoxide, ascorbic acid, glutathione, uric acid, taurine, HUMAN-BLOOD PLASMA, ASCORBATE, ERYTHROCYTES, GENERATION, DEFENSE, OXYGEN
  • Akdeniz University Affiliated: Yes

Abstract

The role of antioxidants in the prevention of t-butyl hydroperoxide-induced chemiluminescence.

Gümüşlü S1, Yücel G, Aydin M, Yeşilkaya A, Demir AY, Aksu TA.

 

 

Abstract

An experimental system which assesses the antioxidant potential of ascorbic acid, glutathione, uric acid, and taurine was developed. The system comprised hemoglobin, luminol, t-butyl hydroperoxide, and different concentrations of antioxidants in TRIS-HCl buffer (pH 7.4). Control assays were performed by excluding antioxidants. Chemiluminescence was detected using a liquid scintillation counter in single photon mode. All antioxidants, when applied in the appropriate concentrations, decreased the maximum chemiluminescence values. The minimum concentrations which decreased the chemiluminescence values were defined for each of the antioxidants.